Purification of proteins

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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530364, 210660, 210668, 435 711, 435 712, C07K 118

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active

056250410

ABSTRACT:
Problem: when proteins are purified using a protein-binding dye immobilised on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. Solution: an ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein.

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