Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-08-30
2005-08-30
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C435S069100
Reexamination Certificate
active
06936699
ABSTRACT:
The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.
REFERENCES:
patent: 5723118 (1998-03-01), Sebald
patent: 6506590 (2003-01-01), Apeler et al.
patent: 0613499 (1994-09-01), None
patent: 1022337 (2000-07-01), None
Hehn et al., Nuclear magnetic resonance imaging of structural changes in skin under phonophoretically enhanced drug permeation, Jul. 1996, Pharmazeutische Industerie, vol. 586, No. 6, Abstarct.
Laurie G W et al., Localization of Binding Sites for Laminin Heparan Sulfate Proteoglycan and Fibronectin on Basement Membrane Type IV Collagen, 1986 Journal of Molecular Biology, vol. 189, No. 1, Abstract.
Marston, F., The Purification of Eukaryotic Polypeptides Synthesized inEscherichia coli, Biochem. J., 240: 1-12 (1986).
Bailey, et al., The Effect of Denaturants on the Crossflow Membrane Filtration ofEscherichia coliLysates Containing Inclusion Bodies, J. Membrane Sci., 131: 29-38 (1997).
Bailey, et al., Crossflow Microfiltration of RecombinantEscherichia coliLysates after High Pressure Homogenization, Biotechnology & Bioengineering, 56(3): 304-310 (Nov., 1997).
Blum, et al., DnaK-Mediated Alterations in Human Growth Hormone Protein Inclusion Bodies, Biotechnology, 10: 301-304 (Mar., 1992).
Bowden, et al., Structure and Morphology of Protein Inclusion Bodies inEscherichia coli, Biotechnology, 9: 725-730 (Aug., 1991).
Meyeroltmanns, R., Membran-Tangenitalflub-Filtrationssysteme, BioTec, 5: 918-921 (Oct., 1991).
Riesenberg, et al., High Cell Density Fermentation of RecombinantEscherichia coliExpressing Human Interferon Alpha 1, Applied Microbiology & Biotechnology, 34: 77-82 (1990).
Schoemaker, et al., Examination of Calf Prochymosin Accumulation inEscherichia coli: Disulphide Linkages Are a Structural Component of Prochymosin-containing Inclusion Bodies, EMBO J., 4(3): 775-780 (1985).
Schoner, et al., Isolation and Purification of Protein Granules FromEscherichia coliCells Overproducing Bovine Growth Hormone, Biotechnology, 151-154 (Feb., 1985).
Walker, S., Lyddiatt, A., “Aqueous Two-Phase Systems as an Alternative Process Route for the Fractionation of Small Inclusion Bodies”, J. of Chromatography B: Biomedical Applications, 711: 185-194 (1998).
Bailey, S., Meagher, M., “Separation of Soluble Protein From Inclusion Bodies inEscherichia coliLysate Using Crossflow Microfiltration”, J. of Membrane Science, 166: 137-146 (2000).
Meagher, M., Barlett, R., Rai, V., Khan, F., “Extraction of rIL-2 Inclusion Bodies FromEscherichia coliUsing Cross-Flow Filtration”, Biotechnology and Bioengineering, 43: 969-977 (1994).
Forman, S., DeBernardez, E., Feldberg, R., Swartz, R., “Crossflow Filtration For the Separation of Inclusion Bodies From Soluble Proteins in RecombinantEscherichia coliCell Lysate”, J. of Membrane Science, 48: 263-279 (1990).
Chem. Abstr. XP-002184947, Graham,E., Howell, J., “Fractionation of Proteins Using Ultrafiltration Membranes Retrieved From STN” (1998).
Sharma, S., Recovery of Soluble Human Renin From Inclusion Bodies Produced in RecombinantEscherichia coli, J. Biotechnology, 4: 119-124 (1986).
Taylor, et al., Size and Density of Protein Inclusion Bodies, Biotechnology, 4: 553-557 (Jun. 1986).
Bayer Aktiengesellschaft
Mondesi Robert B.
Weber Jon
LandOfFree
Purification of protein inclusion bodies by crossflow... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of protein inclusion bodies by crossflow..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of protein inclusion bodies by crossflow... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3461625