Purification of protein a by affinity chromatography followed by

Details Purification of protein a by affinity chromatography followed by Purification of protein a by affinity chromatography followed by

1990-01-24

1991-12-24

Moskowitz, Margaret

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530413, 530416, C07K 318, C07K 322, C07K 328, C07K 1504

Patent

active

050754238

ABSTRACT:
Affinity-purified protein A preparations are contacted with a suitable anionic exchange material in order to remove trace contamination. Staphylococcal enterotoxin B (SEB) and other proteinaceous contaminants are removed by passing such affinity-purified protein A preparations through a DEAE-cellulose column and thereafter selectively eluting the protein A to separate the contaminants. Very high purity protein A composition are thus obtained, typically having a contaminant concentration of about 5 weight % or below, with below about 0.001 weight % SEB.

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