Purification of polypeptides

Details Purification of polypeptides Purification of polypeptides

2001-04-20

2008-09-02

Russel, Jeffrey E (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Separation or purification

C530S300000, C530S345000, C530S402000, C530S408000, C530S409000, C530S410000, C530S812000

Reexamination Certificate

active

07420035

ABSTRACT:
This invention relates to a process for purifying a polypeptide, a capture tag useful for purifying a polypeptide and a periodate-cleavable amino acid derivative useful for purifying a polypeptide. The polypeptide to be purified comprises a vicinal-amino-thiol, vicinal-amino-hydoxyl, vicinal-diol or vicinal-diamino group. The purification process comprises attaching the polypeptide to a purification matrix by contacting the polypeptide with a purification matrix comprising aldehyde or ketone groups under conditions which favor formation of a heterocyclic ring system, washing the purification matrix, and releasing the polypeptide from the purification matrix.

REFERENCES:
patent: 5084555 (1992-01-01), Coy et al.
patent: 5130255 (1992-07-01), Battersby et al.
patent: 5733858 (1998-03-01), Wilson et al.
patent: 5965106 (1999-10-01), Pomato et al.
patent: 88/02776 (1988-04-01), None
Chu et al. Active Site Directed N-Carboxymethyl Peptide Inhibitors . . . Biochemistry. 1984, vol. 23, No. 16, pp. 3598-3603.
S. Narayanan et al., “Glutaraldehyde-P, a Stable, Reactive Aldehyde Matrix for Affinity Chromatography,”Analytical BioChemistry, 188:278-284, 1990.

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