Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-08-06
1998-01-13
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
4351721, 4352523, 4353201, 536 231, C12Q 168, C12N 1563, C12N 1564, C07H 2104
Patent
active
057078122
ABSTRACT:
A method for purifying plasmid DNA during column chromatography is provided. A short chain polymeric alcohol, preferably polyethylene glycol, or another DNA condensation agent, is added to a DNA sample prior to column chromatography. The short chain polymeric alcohol or condensation agent promotes improved isolation of plasmid DNA and may be used for large scale purification, particularly for manufacturing plasmid DNA as a biopharmaceutical.
REFERENCES:
Vasilevskaya, V., et al. (1995) Collapse of single DNA molecule in poly(ethylene glycol) solutions. J. Chem. Phys. 102(16):6595-6602.
Wilson, R., et al. (1979) Counterion-induced condensation of deoxyribonucleic acid. A light-scattering study. Biochemistry 18(11):2192-2196.
Yoshikawa, K., et al. (1996) Nucleation and growth in single DNA molecules. J. Am. Chem. Soc. 118:929-930.
Zhang, W., et al. (1996) Large electrostatic differences in the binding thermodynamics of a cationic peptide to oligomeric and polymeric DNA. Proc. Natl. Acad. Sci. 93:2511-2516.
Manning, G. (1978) The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides. Quarterly Reviews of Biophysics II 2:179-246.
Minagawa, K., et al. (1994) Direct observation of the coil-globule transition in DNA molecules. Biopolymers 34:555-558.
Plum, G., et al. (1990) Condensation of DNA by trivalent cations. 2. Effects of cation structure. Biopolymers 30:631-643.
Post, C., et al. (1982) Theory of DNA condensation: collapse versus aggregation. Biopolymers 21:2123-2137.
Schaper, A., et al. (1993) Scanning force microscopy of circular and linear plasmid DNA spread on mica with a quaternary ammonium salt. Nucleic Acids Research 21(25):6004-6009.
Smith, S., et al. (1996) Overstretching B-DNA: The elastic response of individual double-stranded and single-stranded DNA molecules. Science 271:795-799.
Cheng, S., et al. (1975) Condensed states of nucleic acids. II. Effects of moelcular size, base composition, and presence on intercalating agents of the transition of DNA. Biopolymers 14:663-674.
Eickbush, T., et al. (1978) The compaction of DNA helices into either continuous supercoils or folded-fiber rods and toroids. Cell 13:295-306.
Horn, N., et al. (1995) Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Human Gene Therapy 6:565-573.
Lerman, L.S. (1971) A transition to a compact form of DNA in polymer solutions. Proc. Natl. Acad. Sci. 68(8):1886-1890.
Ma, C., et al. (1995) Condensation of plasmids enhanced by Z-DNA conformation of d(CG).sub.n Inserts. Biochemistry 34:3521-3528.
Ma, C., et al. (1995) Gel electrophoresis measurement of counterion condensation on DNA. Biopolymers 35:211-216.
Akimenko, N.M., et al. (1973) Viscosimetric study on compact form of DNA in water-salt solutions containing polyethylenegycol. FEBS Letters 38(1):61-63.
Al-Shakhshir, R., et al. (1995) Contribution of electrostatic and hydrophobic interactions to the adsorption of proteins by aluminium-containing adjuvants. Vaccine 13(1):41-44.
Anderson, C., et al. (1990) Ion Distributions around DNA and other cylindrical polyions: theroretical descriptions and physical implications. Annu. Rev. Biophys. Chem. 19:423-465.
Anderson, C., et al. (1995) Salt-nucleic acid interactions. Annu. Rev. Phys. Chem. 46:657-700.
Arscott, P., et al. (1990) Condensation of DNA by trivalent cations. 1. Effects of DNA length and topology on the size and shape of condensed particles. Biopolymers 30:619-630.
Arscott, P., et al. (1995) DNA condensation by cobalt hexaammine (III) in alcohol-water mixtures: dielectric constant and other solvent effects. Biopolymers 36:345-364.
Budahazi Greg
Horn Nancy
Marquet Magda
Guzo David
Sandals William
Vical Incorporated
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