Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lipoproteins – e.g. – egg yolk proteins – cylomicrons – etc.
Patent
1986-10-20
1987-07-28
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lipoproteins, e.g., egg yolk proteins, cylomicrons, etc.
435190, 435191, 435197, 435938, 514 2, 514 8, 530371, C07K 302, C07K 1504
Patent
active
046832939
ABSTRACT:
Method for the selective extraction of desired lipophilic proteins from transformed cells of the genus Pichia by cell lysis in the presence of chaotrophic salts is disclosed. The total protein extracted under the invention cell lysis conditions is reduced while the recovery of desired lipophilic proteins remains relatively constant, thereby producing a cell extract with enhanced concentration of the desired lipophilic protein relative to a control cell extract.
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patent: 4604377 (1986-08-01), Fernandes et al.
"Lysis of Halophilic Vibrio Alginolyticus and Vibrio Costicolus Induced by Chaotropic Anions", T. Unemoto et al, Biochimica et Biophysica Acta, 500, pp. 42-431 (1977).
"Multiple Chemical Forms of Hepatitis B Surface Antigen Produced in Yeast", D. E. Wampler et al, Proc. Natl. Acad. Sci. USA, vol. 82, pp. 6830-6834, Oct. 1985.
Phillips Petroleum Company
Reiter S. E.
Schain Howard E.
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