Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1989-04-06
1991-03-05
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
424 92, 435 712, 530380, A61K 3902, A61K 3910, C07K 318, C07K 312
Patent
active
049979159
ABSTRACT:
Lymphocytosis promoting factor (LPF) and filamentous hemaglutinin (FHA) are isolated from the growth medium of the Bordatella pertussis organism and purified by selecting adsorbing the LPF and FHA on a selective adsorbing medium, such as filter aids or gel filtration media, at low ionic strength and subsequently removing the adsorbed LPF and FHA at using an aqueous medium of high ionic strength, either simultaneously or sequentially. Prior to desorbtion of the LPF and FHA, the adsorbing medium may be contacted with an aqueous solution of a non-ionic detergent, which enables the LPF and FHA subsequently desorbed to be substantially free from contamination by lipopolysaccharides (LPS). The LPF and FHA may be further purified on hydroxyapatite. The LPF and FHA may be detoxified separately or together by contacting with a cross-linking agent, such as glutaraldehyde or formaldehyde, in the presence of an anti-aggregation agent. The resulting purified and detoxified LPF and FHA may be used to formulate a vaccine against pertussis.
REFERENCES:
patent: 4455297 (1984-06-01), Syukuda et al.
patent: 4563303 (1986-01-01), Ginnaga et al.
patent: 4687738 (1987-08-01), Ginnaga et al.
patent: 4705686 (1987-11-01), Scott et al.
patent: 4784589 (1988-11-01), Robinson et al.
Alkema Dirk
Jackson Gail
Tan Larry U. L.
Wah Po S.
Connaught Laboratories Limited
Koh Choon P.
Schain Howard E.
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