Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1991-04-12
1994-05-10
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530397, 530413, C07K 320, C07K 1506, C07K 1514
Patent
active
053108809
ABSTRACT:
The present invention discloses a method of purifying Mullerian Inhibiting Substance. The method takes advantage of immunoaffinity chromatography to achieve MIS at up to 95% purity. The method provides for a purified MIS product in which contaminating enzymes having MIS proteolytic activity or inhibitors of MIS antiproliferative activity are substantially removed by addition of an alkali metal halide solution or an alkaline earth metal halide solution prior to elution with an acid solution having a pH of between about 2.5 and 4.0.
REFERENCES:
patent: 4404188 (1983-09-01), Donahoe et al.
patent: 4487833 (1984-12-01), Donahoe et al.
patent: 4510131 (1985-04-01), Donahoe et al.
patent: 4753794 (1988-06-01), Donahoe
patent: 4792601 (1988-12-01), Donahoe et al.
patent: 5010055 (1991-04-01), Donahoe et al.
patent: 5011687 (1991-04-01), Donahoe et al.
patent: 5047336 (1991-09-01), Cate et al.
Harris et al, Protein Purification Methods IRL Press. 1989, pp. 282, 286-289.
Cate et al, "Isolation of the Bovine and Human Genes for Mullerian Inhibiting Substance . . . ". Cell, Jun. 6, 1986. vol. 45, pp. 685-698.
Shima et al, "Production of Monoclonal Antibodies for Affinity Purification of Bovine Mullerian . . . ", Hybridona. 1984. vol. 3(3). pp. 201-214.
Donahoe et al., Science, 205:913-15 (1979).
Pepinsky et al., J. Biol. Chem., 263(35):18961-4 (1988).
Fuller et al., J. Clin. Endocrinol. Metab., 54(5):1051-5 (1982).
Fuller et al., Gynecol. Oncol., 22:135-48 (1985).
Wallen et al., Cancer Res., 49:2005-11 (1989).
Epstein et al., In Vitro Cell. and Devel. Biol., 25(2):213-6 (1989).
Hudson, et al., J. Clin. Endocrinol. Metab., 70(1):16-22 (1990).
Cate et al., "Mullerian-Inhibiting Substance" in Handbook of Experimental Pharmacology, 95/II:179-210 (1990).
Chin et al., Cancer Res., 51 (8):2101-06 (Apr. 15, 1991).
Donahoe Patricia K.
MacLaughkin David T.
Ragin Richard C.
Russel Jeffrey E.
The General Hospital Corporation
LandOfFree
Purification of Mullerian inhibiting substance does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of Mullerian inhibiting substance, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of Mullerian inhibiting substance will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2413124