Purification of monomeric interferon

Details Purification of monomeric interferon Purification of monomeric interferon

1987-10-06

1988-08-23

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 424 85, 435803, 435808, 530351, 530413, 530417, B01D 1508, C07K 1526, A61K 4502

Patent

active

047659033

ABSTRACT:
Fast moving monomers of alpha-2 interferon ("IFN-alpha-2") are purified from slow moving monomers and oligomers of interferon and from noninterferon proteins using a combination of immunoaffinity chromatography, reverse phase HPLC, cation exchange chromatography, and gel filtration. Two fast monomer forms, FMM.sub.1 and FMM.sub.2, are further distinguished on the basis of charge and hydrophobicity and FMM.sub.2 is obtained in essentially homogeneous form with respect to size, hydrophobicity and charge.

REFERENCES:
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