Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1997-06-18
1999-03-23
Tsang, Cecilia J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
530370, 530379, 530402, 530412, 530416, 530417, 530418, 530422, 530427, C07K 100, A23J 100
Patent
active
058861556
ABSTRACT:
A method for purifying a plant protein comprises: (a) preparing a crude plant extract; (b) passing the crude plant extract with a first buffer solution through a guard column comprising a hydrophobic interaction chromatography medium of hydrophobicity sufficiently high to prevent tannins and polyphenols from eluting from the column in the presence of the buffer solution, but sufficiently low that a protein fraction elutes with the first buffer solution; (c) passing the protein fraction through a capture column coupled in series to the guard column, the capture column comprising a hydrophobic interaction chromatography medium of hydrophobicity sufficiently high to prevent the protein from eluting in the presence of the buffer solution; and (d) eluting the protein from the capture column as a purified fraction. Preferably the plant is miracle fruit, the protein is Miraculin, and the method comprises ion exchange chromatography and gel filtration chromatography of the purified fraction of the protein. Foods and beverages may be sweetened with the purified Miraculin.
REFERENCES:
patent: 3676149 (1972-07-01), Fennel et al.
patent: 3925547 (1975-12-01), Henkin et al.
"HiLoad.TM. Phenyl Sepharose.RTM. High Performance," Data File, Pharmacia Biotech. (18-1022-55).
Takahashi et al, The Journal of Biological Chemistry, vol. 265, No. 14, pp. 7793-7798, May, 15, 1990.
"Media For Hydrophobic Interaction Chromatography," Data File, Pharmacia Biotech.
"HiTrap.RTM. HIC Test Kit," Data File, Pharmacia Biotech.
"HiLoad.TM. Phenyl Sepharose.RTM. High Performance," Data File, Pharmacia Biotech (18-1022.55).
"Resource.RTM. ETH, Resource.RTM. ISO, Resource.RTM. PHE," Data File, Pharmacia Biotech.
"Phenyl Sepharose.RTM. 6 Fast Flow (low sub) Phenyl Sepharose 6 Fast Flow (high sub)," Data File 2040, Pharmacia Biotech, 1991.
Kurihara, "Taste-Modifying Protein from Miracle Fruit," Science, vol. 161, 1968, pp. 1241-1243.
Brouwer et al., "Miraculin, the Sweetness-inducing Protein from Miracle Fruit," Nature, vol. 220, Oct. 1968, pp. 373-374.
E. L. Giroux et al., "Purification and Some Properties of Miraculin, a Glycoprotein from Sunsepalum dulcificum Which Provokes Sweetness and Blocks Sourness," J. Agr. Food Chem., vol. 22, No. 4, 1974, pp. 596-600.
F.R. Dastoli et al., "Miracle-Fruit Concentrate," Sweeteners, Ch. 18, pp. 205-210.
N. Pintauro, "Miraculin, Glycyrrhizin and Artichoke Sweeteners," Sweeteners and Enhancers, Food Technology Review 190 40, Noyes Data Corp. 1977.
S. Theerasilp et al., "Complete Purification and Characterization of the Taste-modifying Protein, Miraculin, from Miracle Fruit," J. Biol. Chem., vol. 263, No. 23, pp. 11536-11539, Aug. 1988.
Y. Kurihara et al., "Isolation and Chemical Properties of Multiple Active Principles From Miracle Fruit," Biochimica et Biophysica Acta. 719, pp. 444-449, Elsevier Biomedical Press, 1982.
Achel Daniel Gyingiri
Acquaah, deceased Robert Asare
Armah George Enyimah
Belew Makonnen
BioResources International Inc.
Gollin Michael A.
Mohamed Abdel A.
Pharmacia Biotech AB
Tsang Cecilia J.
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