Purification of microbial rennet from Mucor miehei

Details Purification of microbial rennet from Mucor miehei Purification of microbial rennet from Mucor miehei

1984-11-05

1988-05-10

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435815, C12N 964

Patent

active

047435518

ABSTRACT:
A method for the purification of a culture filtrate resulting from the fermentation of an organism of the species Mucor miehei involves the selective adsorption of the microbial rennet present in the culture filtrate by a blue dye affinity ligand with subsequent elution of the adsorbed microbial rennet to provide it in a purified form.

REFERENCES:
patent: 4666843 (1987-05-01), Subramanian
Kobayaski et al, Analytical Biochemistry, 122, 308-312 (1982).
Subramanian, CRC Critical Reviews in Biochemistry, vol. 16, (1982).
Issue 2, pp. 169-205, Dean et al, J. Chromatogs, 165, 301 (1979).
Burgett et al, Am. Lab., p. 74 (1977).

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