Purification of lipoprotein cholesterol for use as a cholesterol

Details Purification of lipoprotein cholesterol for use as a cholesterol Purification of lipoprotein cholesterol for use as a cholesterol

1980-01-07

1981-09-22

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

252408, 260112B, G01N 3368, G01N 3392, G01N 3396

Patent

active

042907748

ABSTRACT:
A method for the purification of lipoprotein cholesterol. The method involves the steps of adsorbing lipoprotein material on a silica adsorbant, eluting the adsorbed lipoprotein with water at a pH of about 10 to 11.5, adjusting the salt concentration to about less than 0.05M, heating the lipoprotein to about 50.degree. to 100.degree. C. for a period of about 5 minutes to 24 hours, adding an alkaline carbonate and alkaline earth salt to form a precipitate and removing the precipitate, adjusting the pH from about 6.5 to 9.0 and recovering purified lipoprotein cholesterol. Also a method for using the cholesterol as a cholesterol reference material.

REFERENCES:
patent: 4096136 (1978-06-01), Ayers
patent: 4110077 (1978-08-01), Klein
W. Stephan et al., Z. Klin. Chem. Klin. Biochem., 6(3), 186-190 (1968).
W. Werner, J. Chromatog, 25(1), 63-70 (1966).
Chemical Abstracts, 69:17412h (1968).

Affiliated with

Also associated with

Rating

Purification of lipoprotein cholesterol for use as a cholesterol does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of lipoprotein cholesterol for use as a cholesterol, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of lipoprotein cholesterol for use as a cholesterol will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-333152