Purification of interferon

Details Purification of interferon Purification of interferon

1979-10-12

1981-07-14

Phillips, Delbert R.

Drug, bio-affecting and body treating compositions

Lymphokine

435811, A61K 4502

Patent

active

042786614

ABSTRACT:
Human interferons produced in the absence of added serum or sera can be purified to greater than 95% protein purity by adsorption on immobilized Cibacron Blue F3G-A and eluting the interferon adsorbed on the Cibacron Blue F3G-A with ethylene glycol in an aqueous buffer solution. The purified interferon in solution can be converted to interferon of uniform molecular weight by heating the interferon solution in the presence of an organic thiol compound.

REFERENCES:
patent: 4172071 (1979-10-01), De Maeyer et al.
Jankowski, W., et al., Biochemistry, vol. 15, pp. 5182-5187, (1976).
Bollin, E., et al., Preparative Biochemistry, vol. 8, pp. 259-274, (1978).
Erickson, J., et al., Interferon Scientific Memoranda, Mar. 1979.
Erickson, J., et al., Analytical Biochemistry, vol. 98, pp. 214-218, (1979).
Osario, T., et al., Proc. Soc. Exp. Biol. Med., vol. 153, pp. 484-489, (1976).
Maeyer-Guignard et al., C. R. Acad. Sc. Paris, vol. 283, pp. 709-711, (1976).
Davey, M., et al., Biochemistry, vol. 15, pp. 5182-5187, (1976).
Sulkowski et al., J. Biol. Chem., vol. 251, pp. 5381-5385, (1976).
Stewart, W., Editor, CRC Press, Inc., Cleveland, Ohio, "Interferon," pp. 49-72, (1977).
"Blue Sepharose CL-6B For Affinity Chromatography," Sep. 1976, Pharmacia Fine Chemicals, publisher.

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