Purification of human Troponin I

Details Purification of human Troponin I Purification of human Troponin I

2005-01-11

2005-01-11

Carlson, Karen Cochrane (Department: 1653)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Chemical modification or the reaction product thereof, e.g.,...

C530S412000, C530S335000, C435S069100, C435S252300, C435S320100, C435S471000, C435S476000, C536S023100, C536S023500, C536S024200

Reexamination Certificate

active

06841658

ABSTRACT:
The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

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