Purification of human myelomonocyte interferon gamma with an imm

Details Purification of human myelomonocyte interferon gamma with an imm Purification of human myelomonocyte interferon gamma with an imm

1996-04-01

1997-09-30

Naff, David M.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

424 855, 435 7021, 435 705, 4352402, 43524026, 436518, 436548, 530351, C07K 320, C07K 1700, C12P 2104, G01N 3353

Patent

active

056726929

ABSTRACT:
A human myelomonocyte interferon-gamma having a novel polypeptide and carbohydrate chain structure is produced by propagation of an established human myelomonocyte in vitro or after being implanted in a non-human warm-blooded animal or in a diffusion chamber placed inside or outside the body of the animal. The human myelomonocyte may be contacted with an inducer during propagation. A monoclonal antibody specific to the human myelomonocyte interferon-gamma is produced by immunizing a non-human warm-blooded animal with purified human myelomonocyte interferon-gamma as an antigen, recovering an antibody producing cell from the animal and fusing the cell with a myeloma cell to produce a hybrid capable of producing the monoclonal antibody. The human myelomonocyte interferon-gamma can be purified by chromatography with an immobilized anti-human myelomonocyte interferon-gamma antibody such as the monoclonal antibody. The human myelomonocyte interferon-gamma can be used as a prophylactic and therapeutic agent for human interferon-gamma susceptive diseases.

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Devos et al, J. Interferon Res., 4, 461-468 (1984).
Yamamoto et al, Studies on the Sugar Chains of Interferon from Human Peripheral-Blood Lymphocytes, J. Biochem., 105:1034-1039 (1989).

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