Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1990-05-31
1991-12-31
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
424 852, 435 6952, C07K 328
Patent
active
050773887
ABSTRACT:
Active recombinant human IL-4 is purified from a crude fermentation broth of a secreted E. coli mutant by subjecting said solution to selective chromatogrphy on a zinc-chelating agarose column at pH 7.2 in a buffer containing a high concentration of sodium chloride, i.e., about 1M sodium chloride, washing first with a phosphate buffer at pH 7.2 containing 1.0M sodium chloride then with a buffer containing 10% glycerol and eluting at pH 5.0. The resulting purified active recombinant human IL-4 solution is further treated by cation exchange chromatography, concentration by diafiltration up to 20 mg/mL and size exclusion gel chromatography using a citrate buffer at pH 4.5.
REFERENCES:
Dwyer, Biotechnology, 2, 957 (1984).
Yokota, et al., Proc. Natl. Acad. Sci. U.S.A. 83, 5894-5898 (1986).
Lee et al., Proc. Natl. Acad, Sci., U.S.A., 83, 2061-2065 (1986).
Noma, et al., Nature 319, 640-646 (1986).
Nagabhushan Tattanahali L.
Naveh David
Tang John C. T.
Dulak Norman C.
Nelson James R.
Rosen Gerald S.
Schain Howard E.
Schering Corporation
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