Purification of human interleukin-4 from a CHO-cell line culture

Details Purification of human interleukin-4 from a CHO-cell line culture Purification of human interleukin-4 from a CHO-cell line culture

1990-07-26

1991-07-23

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 514 2, 530351, 530413, 530416, 530417, B01D 1508

Patent

active

050341332

ABSTRACT:
Active recombinant human IL-4 is purified from a crude cell culture medium of a CHO-cell lines mutant by subjecting said crude solution to two sequentialcation exchange chromatographies at pH 7.2 and 0.12M sodium chloride, selective chromatography on a cobalt-chelating agarose column at pH 7.2 in a buffer containing a high concentration of sodium chloride, i.e., about 0.5M sodium chloride, further treated by concentration (diafiltration) up to 20 mg/mL and size exclusion gel chromatography using a citrate buffer at pH 4.5.

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