Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1993-03-05
1994-07-12
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
435 6952, 435 704, 530415, 530416, C07K 302, C07K 320, C07K 322, C07K 1506
Patent
active
053289897
ABSTRACT:
A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL-10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.
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Mosmann et al "Isolation of Monoclonal Antibodies Specific For 12-4, 12-5, 12-6 and a New ThZ Specific Cytokine (11-10), Cytokine Synthesis Inhibitory Factor, By Using A Solid Phase Radioimmunoadsorbent Assay"J. Immunol. 145: 9 2938-2945 Nov. 1, 1991.
Cannon-Carlson Susan
Tang John
Vellekamp Gary
Dulak Norman C.
Gromet Nancy J.
Kanstad Steinar V.
Lunn Paul G.
Russel Jeffrey E.
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