Purification of human interleukin-10 from a cell culture medium

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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435 6952, 435 704, 530415, 530416, C07K 302, C07K 320, C07K 322, C07K 1506

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active

053289897

ABSTRACT:
A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL-10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.

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Guide to Protein Purification Murray Deutscher (Ed) Academic, Press Inc. (1990).
Ingley, et al. "Production & Purification of recombinant human interleukin-S from yeast & baculovhus expression systems". Eur. J. Biochem 196 623-629. 1991.
Mosmann et al "Isolation of Monoclonal Antibodies Specific For 12-4, 12-5, 12-6 and a New ThZ Specific Cytokine (11-10), Cytokine Synthesis Inhibitory Factor, By Using A Solid Phase Radioimmunoadsorbent Assay"J. Immunol. 145: 9 2938-2945 Nov. 1, 1991.

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