Purification of human chorionic gonadotropin .beta.-core molecul

Details Purification of human chorionic gonadotropin .beta.-core molecul Purification of human chorionic gonadotropin .beta.-core molecul

1991-11-12

1995-08-29

Saunders, David

Chemistry: analytical and immunological testing

Immunochemical pregnancy determination

436518, 436536, 436547, 436548, 436808, 436813, 436814, 436818, 53038824, 5303892, 5303913, C07K 1626, G01N 33531

Patent

active

054459680

ABSTRACT:
The present invention relates to the purification of the human chorionic gonadotropin .beta.-core molecule which can then be used as the antigen in the preparation of antibodies to the .beta.-core molecule. The combination of the purified .beta.-core molecule and the antibodies can be used in an immunoassay kit to measure .beta.-core molecules in the presence of structurally similar molecules, i.e., hCG, LH, hCG.beta.-subunit and LH.beta.-subunit. Measurement of the .beta.-core molecule is particularly useful in testing for pregnancy and many malignancies.

REFERENCES:
patent: 4565687 (1986-01-01), Khazaeli et al.
patent: 4804626 (1989-02-01), Bellet et al.
patent: 4851356 (1989-07-01), Canfield et al.
patent: 5356817 (1994-10-01), Cole
Kohler et al (1975) Continuous cultures of fused cells . . . Nature 256: 495-497.
Schroeder et al (1983) Specificity of Human . . . Clin Chem 29(4): 667-671.
Blithe et al (1988, Jan. (Purification of .beta.-Core Fragment . . . Endocrinology 122: 173-180.
O'Connor et al; Cancer Research vol. 48(5) Mar. 1, 1988 pp. 1361-1366.
Abstract Nos. B2.144.09, B2.144.10, 15th International Cancer Congress, Aug. 16-22, 1990.
Alfthan et al; Jo. Clin. Endrocin. & Metabolism. vol. 70, No. 3, Mar. 1990. pp. 783-787.
Akar et al; Jo. Clin. Endocrin & Metabolism vol. 66, No. 3; Mar. 1988 pp. 538-545.
D. G. Smyth, Methods in Enzymology, vol. 11, pp. 214 (1967).
L. J. Greene and D. C. Bartlett, Methods in Enzymology, vol. XLVII, p. 170 (1977).
R. J. Ryan et al., "Structure-function Relationships of Gonadotropins", Recent Progress in Hormone Research, vol. 43, p. 83 (1987).
B. Shome and A. F. Parlow, J. Clin. Endo. and Metab., 36, 618 (1973).
Birken and R. E. Canfield, J. Biol. Chem. 252, 5386-92 (1977).
Birken et al., "Structure of the Human Chorionic Gonadotropin B-Subunit Fragment from Pregnancy Urine", Endocrinology, vol. 123, No. 1, pp. 572-583 (1988).
Kardana et al., "Urinary Gonadotrophin Peptide-Isolation and Purification, and its Immunohistochemical Distribution in Normal and Neoplastic Tissues", British Journal of Cancer, vol. 58, No. 3, Sep. 1988.
Cole et al., "Urinary Human Chorionic Gonadotripin Free B-Subunit and B-Core Fragment: A New Marker of Gynecological Cancers", Cancer Research 48, pp. 1356-1360, Mar. 1, 1988.
Krichevsky et al., "Preparation and Characterization of Antibodies to the Urinary Fragment of the Human Chorionic Gonadotropin B-Subunit", Endocrinology, vol. 123, No. 1, pp. 584-593 (1988).

Affiliated with

Also associated with

Rating

Purification of human chorionic gonadotropin .beta.-core molecul does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of human chorionic gonadotropin .beta.-core molecul, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of human chorionic gonadotropin .beta.-core molecul will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1819102