Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...
Patent
1987-04-14
1988-05-03
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Plant proteins, e.g., derived from legumes, algae or...
424 88, 435 68, 43524027, 530350, 530380, 530413, 530806, 530808, 530826, C07K 320, C07K 328, A61K 3929
Patent
active
047421584
ABSTRACT:
Recombinant pre-S-HBsAg is purified by a rapid and efficient two step chromatographic process. Yeast cells expressing recobinant pre-S-HBsAg are disrupted, the cell contents are clarified and separated by polymerized human serum albumin affinity chromatography. The pre-S-HBsAg is further purified by hydrophobic interaction chromatography using butyl agarose. This process results in pre-S-HBsAg that is greater than 90% pure.
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Lehman E. Dale
McAleer William J.
Schaefer Ted F.
Merck & Co. , Inc.
Perrella Donald J.
Schain Howard E.
Speer Raymond M.
Tribble Jack L.
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