Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1989-08-17
1992-05-19
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
A61K 3514, C07K 1300
Patent
active
051151006
ABSTRACT:
A method for purifying hemoglobin, mixed with other proteins is described, wherein the mixture is contacted with an ion exchange matrix so that the hemoglobin is adsorbed to it. The matrix is washed to remove unadsorbed components, and the hemoglobin is then selectively eluted using a ligand which specifically binds the hemoglobin causing it to desorb from the matrix. The method can also be used to effect reduction of methemoglobin to hemoglobin on an ion exchange matrix followed by selective elution to achieve purification. The method can also be used to purify methemoglobin.
REFERENCES:
patent: 4407961 (1983-10-01), Sanders
patent: 4600531 (1986-07-01), Walder
Hsia et al. (1986), Purification of Stroma-free Haemoglobin by ATP-agarose Affinity Chromatography, J. Chrom. 374:143-148.
Wong Jeffrey T.
Wu Xiang-Fu
Ekstrom Richard C.
Kinetic Investments Limited
Schain Howard E.
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