Purification of hemoglobin

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530412, 530413, 530416, 514 6, C07K 122, C07K 118

Patent

active

058408517

ABSTRACT:
The present invention generally relates to methods for purifying hemoglobin solutions and to hemoglobin solutions obtained by the methods. In one aspect, such methods include removing contaminants in crude hemoglobin-containing lysates with heat treatment. In a further aspect, the present invention provides methods for producing substantially purified hemoglobin solutions using immobilized metal affinity chromatography, optionally following by anion exchange chromatography.

REFERENCES:
patent: 4301144 (1981-11-01), Iwashita et al.
patent: 4321259 (1982-03-01), Nicolau et al.
patent: 4336248 (1982-06-01), Bonhard
patent: 4377512 (1983-03-01), Ajisaka et al.
patent: 4401652 (1983-08-01), Simmonds et al.
patent: 4412989 (1983-11-01), Iwashita et al.
patent: 4439357 (1984-03-01), Bonhard et al.
patent: 4473494 (1984-09-01), Tye
patent: 4473563 (1984-09-01), Nicolau et al.
patent: 4526715 (1985-07-01), Klothe
patent: 4598064 (1986-07-01), Walder
patent: 4600531 (1986-07-01), Walder
patent: 4650786 (1987-03-01), Wong
patent: 4670417 (1987-06-01), Iwasaki et al.
patent: 4710488 (1987-12-01), Wong
patent: 4723000 (1988-02-01), Georgiades et al.
patent: 4831012 (1989-05-01), Estep
patent: 4857636 (1989-08-01), Hsia
patent: 4861867 (1989-08-01), Estep
patent: 4952684 (1990-08-01), Yalpani et al.
patent: 4975246 (1990-12-01), Charm
patent: 5028588 (1991-07-01), Hoffman et al.
patent: 5077388 (1991-12-01), Tang et al.
patent: 5084558 (1992-01-01), Rausch et al.
patent: 5115100 (1992-05-01), Wu et al.
patent: 5136026 (1992-08-01), Romisch et al.
patent: 5169936 (1992-12-01), Staples et al.
patent: 5194590 (1993-03-01), Sehgal et al.
patent: 5264555 (1993-11-01), Shorr et al.
patent: 5310648 (1994-05-01), Arnold et al.
patent: 5510247 (1996-04-01), Komives et al.
patent: 5545328 (1996-08-01), Pliura et al.
Kato et al., `High-performance metal chelate affinity chromatography of proteins`, Journal of Cromatography, 354, pp. 511-517, 1986.
Fogh-Andersen, N. et Al/Diode-Array Spectrophotometry for Simultaneous Measurement of Hemoglobin Pigments/Clinica Chimica Acta/(1987) 166: 283-289.
Beguin, P. et Al/Identification of the Endoglucanase Encoded by the celB Gene of Clostridium Thermocellum/Biochimie/(1983) 65: 495-500.
Figueroa, A. et Al/High-Performance Immobilized-Metal Affinity Chromatography of Proteins on Iminodiacetic Acid Silica-Based Bonded Phases/J. of Chromatography/(1986) 371: 335-352.
Zwart, A. et Al/Multicomponent Analysis of Hemoglobin Derivatives with a Revesed-Optics Spectrophotometer/Clin. Chem./(1984) 30: 373-379.
HSIA & SWEE ER/Purification of Stroma-Free Haemoglobin by ATP--Agarose Affinity Chromatography/J. of Chromatography/(1986) 374:143-148.
ESTEP et al/The Purification Hemoglobin Solutions by Heating/Progress in Clinical and Biological Research/The Red Cell Seventh Ann Arbor Conference/(1989) 319: 325-338/ed. George J. Brewer/Alan R. Liss, Inc. New York.
Van Assendelft, O.W. & ijlstra, W.G./Extinction Coefficients for use in Equations for the Spectrophotometric Analysis of Haemoglobin Mixtures/Anal. Biochem./(1975)/69: 43-48.
Antonini, E. & Brunori, M./Hemoglobin and Myoglobin in their Reactions with Ligands/Frontiers of Biology/Structure-Function Relationships in Hemoglobin and Myoglobin/(1971)/21: Chapter 13/348-380/North-Holland Publishing Co./Ed. A. Neuberger & E.L. Tatum.
Antonini, E. & Brunori, M./Hemoglobin and Myoglobin in their Reactions with Ligands/Frontiers of Biologyspecific aspects of the Reactions of Hemoglobin with Ligands/(1971)/21: Chapter 10/235-285/North-Holland Publishing Co./Ed. A. Neuberger & E.L. Tatum.
Benesch, R.E. et al./Equations for the Spectrophotometric Analysis of Hemoglobin Mixtures/Anal. Biochem./(1973)/55: 245-248.
Chang, T.M.S./Modified Hemoglobin: Endotoxin and Safety Studies/Biomat., Art. Cells, Art. Org.,/(1990)/18(2): VII-VIII.
Collison, H.A. et al./Determination of Carbon Monoxide in Blood by Gas Chromatography/Clin. Chem./(1968)/14(2): 162-171.
Commins, B.T. & Lawther, P.J./A Sensitive Method for the Determination of Carboxy-Haemoglobin in a Finger Prick Sample of Blood/Brit. J. Industr. Med./(1965)/22: 139-143.
Darnall, D.W. et al./Selective Recovery of Gold and Other Metal Ions from an Algal Biomass/Environ. Sci. Technol./(1986) 20: 206-208.
Di Iorio, E.E./Preparation of Derivatives of Ferrous and Ferric Hemoglobin/Methods in Enzymology/(1981)/76: 57-72/Academic Press/ Ed. E. Antonini/L. Rossi-Bernardi, E. Chiancone.
Evylyn, K.A. & Malloy, H.T./Microdetermination of Oxyhemoglobin, Methemoglobin, and Sulfhemoglobin, in a Single Sample of Blood/The J. of Bio. Chem./(1938)/126: 655-662.
Figura, P. & McDuffie, B./Characterization of the Calcium Form of Chelex-100 for Trace Metals Studies/Analytical Chemistry/(1977)/49(13): 1950-1953.
Heindorff, K. et al./Genetic Toxicology of Ethylenediaminetetraacetic Acid (EDTA)/Mutation Research/(1983)/115: 149-173.
Johansson, M.-B. & Wollmer, P./Measurement of Carboxyhaemoglobin by Spectrophoto-Metry and Gas Chromatography/Clinical Physiology/(1989)/9: 581-586.
Kato, Y. et al./High-Performance Metal Chelate Affinity Chromatography of Proteins/J. of Chromatography/(1986) 354: 511-517.
Kikugawa, K. et al./Factors Influencing the Autoxidation of Hemoglobin A/Chem. Pharm. Bull./(1981)/29(5): 1382-1389.
Liebhaber, S.A. et al./Cloning and Complete Nucleotide Sequence of Human 5'-.alpha.-Globin Gene/PNAS/(1980)/77(12): 7054-7058.
Marotta, C.A. et al./Human .beta.-Globin Messenger RNA/J. of Biological Chem./(1977)/252: 5040-5053.
Marshall, T. et al./Trace Element Analyses of Diaspirin Cross-Linked Hemoglobin Solutions/Blood Substitutes and Oxygen Carriers/(1993)/Marcel Dekker, Inc./Ed. Chang. T.M.S.
Moyers, E.M. & Fritz, J.S./Preparation and Analytical Applications of a Propylenedia-Minetetraacetic Acid Resin/Anal. Chem./(1977)/49(3)/418-423.
Rabiner, S.F. et al/Evaluation of a Stroma-Free Hemoglobin Solution for use as a Plasma Expander/J. Experimental Med./(1967)126: 1127-1142.
Rietschel, E.T. & Brade, H./Bacterial Endotoxins/Scientific American/(1992)/267: 54-61.
Rodkey, F.L. et al./Spectrophotometric Measurement of Carboxyhemoglobin and Methe-Moglobin in Blood/Clin. Chem./(1979)/25(8): 1388-1393.
Small, K.A. et al/A Rapid Method for Simultaneous Measurement of Carboxy-and Methe-Moglobin in Blood/J. of Applied Physiology/(1971)/31(1): 154-160.
Suffredini, A.F. et al/The Cardiovascular Response of Normal Humans to the Admini-stration of Endotoxin/The New England J. of Med./(1989)/321(5): 280-287.
Tanaka, T. et al/Cloning of 3-Isopropylmalate Dehydrogenase Gene of an Extreme Themo-Phile Purification of the Gene Product/Biochemistry/(1981)/89: 677-682.
Tentori, L. & Salvati, A.M./Hemoglobinometry in Human Blood/Methods in Inzymology/(1981)/76: 707-715/Ed. Antonini, E; Rossi-Bernardi, L.; Chiancone, E./Academic Press
Tsukagoshi, N. et al/Cloning and Expression of a Thermophilic .alpha.-Amylase Gene from Bacillus Stearothermophilus in Escherichia Coli/Mol. Gen Genet/(1984)/193: 58-63.
Vernon, P./Novel Ion Exchangers-The Preparation and Properties of Chelating ion Ex-Change Resins/Chem. & Industry/(1977)/15: 634-637.
White, C.T. et al/Synergistic Toxicity of Endotoxin and Hemoglobin/J. Lab. Clin. Med./(1986) 108(2): 132-137.
Wuenschell, G.E. et al/Aqueous Two-Phrase Metal Affinity Extraction of Heme Proteins/Bioprocess Engineering/(1990)/5: 199-202.
Yip, T-T. et al/Evaluation of the Interaction of Peptides with Cu(II), Ni(II), and Zn(II) by High-Performance Immobilized Metal Ion Affinity Chromatography/Anal. Biochem./(1989)/183: 159-171.
Zijlstra, W.G. et al/Absorption Spectra of Human Fetal and Adult Oxyhemoglobin, De-Oxyhemoglobin, Carboxyhemoglobin, and Methemoglobin/Clin. Chem./(1991)/37(9): 1633-1638.
Chemical Engineering Handbook/5th Edition/(1973)/6/29-6/32/McGraw W-Hill/New York/Ed. R.H. Perry/C.H. Chilton.
Lindeberg, G. et al/Purification of Synthetic Peptides/Int.J. Peptide Protein Res./(1991) 38: 253-259.
Figueroa, A. et al./High-Performance Immobilized-Metal Affinity Chromatography of Proteins on Iminodiacetic Acid Silica-Based Bonded Phases/J. of Chromatography/(1986) 371: 335-352.
Leach, C

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Purification of hemoglobin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of hemoglobin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of hemoglobin will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1703515

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.