Purification of G-CSF

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530395, 530412, 530414, 530413, 530415, 530416, 530417, 530418, 530419, 530420, 530820, 530827, 435 695, 435 701, 435 703, C07K 324, C07K 328

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050555553

ABSTRACT:
A process for purifying human G-CSF from an aqueous solution including the step of adding NaCl to the solution to selectively precipitate the human G-CSF.

REFERENCES:
patent: 4785079 (1988-11-01), Gospodarowicz
Welte et al., PNAS, 82, 1985, pp. 1526-1530.
Pharmacia Bulletin, #50-01-339, 1986, pp. 1-7.
Nicola et al., Nature, 314, 4/85, pp. 625-628.
Nicola et al., Methods of Enzymology, 116, 1985, pp. 600-619.
Neumeier et al., Hoppe-Seyler's Z. Physiol. Chem., 1982, pp. 1493-1500.
Robert K. Scopes, Protein Purification: Principles and Practice, New York: Springer-Verlag, 1982, pp. 39-66.
White, Handler and Smith, Principles of Biochemistry, pp. 122-125.
Watson et al., "Purification of Homogeneity of a Human Hematopoietic Growth Factor that Stimulates the Growth of a Murine Interleukin 3-Dependent Cell Line", J. Immunol., 137:854-857, (1986).
Nomura et al., "Purification and Characterization of Human Granulocyte Colony-Stimulating Factor (G-CSF)", EMBO J., 5:871-876, (1986).

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