Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1985-02-08
1986-03-25
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
424101, C07G 700, A61K 3516, A61K 3514
Patent
active
045782180
ABSTRACT:
Factor VIII is purified by adsorption onto a insoluble matrix having free sulphate groups, such as dextran sulphate, and selective elution therefrom.
A suitable eluant for the purification of the von Willebrand protein (Factor VIIIR:vWp) is citrate buffer, pH 6.85, containing 0.47 M sodium chloride and 2.14 mM calcium chloride.
A suitable eluant for the purification of the Factor VIII complex (Factor VIIIR:Ag, Factor VIIIR:vWp and Factor VIII:C) is citrate buffer at pH value between 6.2 and 7.3 containing 1.0 M glycine, 2.14 mM calcium chloride and 0.5 M sodium chloride at +4.degree. C.
REFERENCES:
patent: 3920625 (1975-11-01), Andersson et al.
patent: 4022758 (1977-05-01), Andersson et al.
patent: 4210580 (1980-07-01), Amrani
patent: 4278594 (1981-07-01), Amrani
patent: 4361509 (1982-11-01), Zimmerman et al.
patent: 4395396 (1983-07-01), Eibl et al.
patent: 4397841 (1983-08-01), Johnson
patent: 4471112 (1984-09-01), Johnson
patent: 4522751 (1985-06-01), Linnau et al.
Introduction to Modern Biochemistry, 3rd Ed. Karlson (1970), pp. 326-327.
Saundry Richard H.
Savidge Geoffrey F.
Schain Howard E.
The Special Trustees for St. Thomas' Hospital
LandOfFree
Purification of factor VIII with insoluble matrix having free su does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of factor VIII with insoluble matrix having free su, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of factor VIII with insoluble matrix having free su will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-629441