Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1992-07-14
1994-02-15
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530384, 530413, 530417, 530420, 530427, A61K 3516, A61K 3702, C07K 320
Patent
active
052868494
ABSTRACT:
The present invention relates to a process for purifying Factor IX from an impure protein fraction containing Factor IX. The purification process comprises the steps of adding a solvent and a detergent to an impure protein fraction and incubating the solvent/detergent protein solution to inactivate any viral contaminants. Factor IX is purified from the solvent/detergent protein solution by chromatography on a sulfated polysaccharide resin without first removing the solvent and detergent prior to the purification on the sulfated polysaccharide resin. The Factor IX, purified by the process has a specific activity of at least 85 units/mg.
REFERENCES:
patent: 4725673 (1988-01-01), Herring
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Bajaj et al., "A Simplified Procedure for Purification of Human Prothrombin, Factor IX and Factor X," Prep. Biochem., 11, 397-412 (1981).
Andersson et al., "Purification and Characterization of Human Factor IX," Thromb. Res., 7, 451-459 (1975).
Menache et al., "Coagulation Factor IX Concentrate: Method of Preparation and Assessment of Potential In Vivo Thrombogenicity in Animal Models," Blood, 64, 1220-1227 (1984).
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Alpha Therapeutic Corporation
Schain Howard E.
Touzeau P. Lynn
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