Purification of DNA with silicon tetrahydrazide

Details Purification of DNA with silicon tetrahydrazide Purification of DNA with silicon tetrahydrazide

1991-10-31

1994-07-12

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435274, 935 19, 935 20, C07H 106, C07H 2100, C07H 2102, C07H 2104

Patent

active

053290003

ABSTRACT:
A process for purifying DNA in which the DNA is bound to silicon tetrahydrazide in the presence of less than 2M chaotrope, low salt buffers, or water. The DNA is then eluted with low salt buffer or by heating in water.

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Louise H. Lutze et al., Nucleic Acids Research 18:6150 (1990).
R. Boom et al., Journal of Clin. Microbiology 28:495 (1990).
Bert Vogelsteil et al., Proc. Natl. Acad. Sci. 76:615 (1979).
Erik H. Willis et al., BioTechniques 9:92 (1990).

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