Purification of DNA on Hydroxylated Silicas

Details Purification of DNA on Hydroxylated Silicas Purification of DNA on Hydroxylated Silicas

1994-11-17

1997-12-02

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 911, 435975, 423335, C12P 1934, C07H 2100, C07H 2104

Patent

active

056937857

ABSTRACT:
Compositions and processes for isolation or purification of DNA are provided. The compositions are hydroxylated silica polymers produced by reacting silicon dioxide with an alkaline solution, followed by acidification. The hydroxylated silicas produced by this process can be used to bind DNA in aqueous solutions, without the need for binding reagents such as alcohols or chaotropes. The bound DNA may then be separated from the solution and eluted into water or a buffer by heating.

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