Purification of DNA on aluminum silicates, and phosphosilicates

Details Purification of DNA on aluminum silicates, and phosphosilicates Purification of DNA on aluminum silicates, and phosphosilicates

1994-03-11

1996-06-04

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 911, C07H 106, C07H 2104, C12N 1510

Patent

active

055233929

ABSTRACT:
A process for purifying DNA comprising binding the DNA to a silicate compound in the presence of water, low salt buffer, or physiological buffer and eluting in the bound DNA by heating in a low salt buffer, a physiological buffer, or water. The silicate compound is prepared by reacting SiCl.sub.4 with a molar percentage of BCl.sub.3 of about 33.5%, 55.6%, or 83.3%, with a molar percentage of PCl.sub.3 of about 50.0%-83.3%, or with a molar percentage of AlCl.sub.3 of about 9.1%; cooling the reaction mixture; adding water until the evolution of gas is complete; and recovering the compound.

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