Purification of deoxyribonuclease

Details Purification of deoxyribonuclease Purification of deoxyribonuclease

1976-10-08

1977-12-27

Naff, David M.

Textiles: fiber preparation

195 63, 195 68, C07G 702, C07G 7026

Patent

active

040653552

ABSTRACT:
Highly purified deoxyribonuclease is obtained by a simple process which comprises adsorption of deoxyribonuclease from a crude or partially purified solution of the enzyme on a column of concanavalin A-agarose, followed by elution of the adsorbed deoxyribonuclease with a carbohydrate solution and recovery of the deoxyribonuclease in solid form from such solution.

REFERENCES:
patent: 3836433 (1974-09-01), Wirth et al.
Avrameas et al., Biologically Active Water-Insoluble Protein Polymers, Their Use for the Isolation of Specifically Interacting Proteins, Biochimie, vol. 53, 1971 (pp. 603-614).
Murthy, et al., Purification of Human & -Antitrypsin by Affinity Chromatography on Sepharose Bound Concenavalina, Febs. Letters, vol. 32, No. 2, 1973, (pp. 243-246).
Fletcher, et al., Protection of Deoxyribonuclease from Ionizing Radiation by Adsorbents, Nature vol. 176, 1955, (pp. 882-883).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, (pp. 28-32 & 79).

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