Purification of d-aminoacid oxidase

Details Purification of d-aminoacid oxidase Purification of d-aminoacid oxidase

1975-04-16

1976-03-02

Shapiro, Lionel M.

Textiles: fiber preparation

195 68, 195DIG11, C07G 702

Patent

active

039416577

ABSTRACT:
Process for the separation and purification of d-aminoacid oxidase by affinity chromatography. The process comprises contacting a mixture of proteins containing the d-aminoacid oxidase with a mineral carrier carrying haloalkylsilane grafts, upon which are fixed -- NH -- (CH.sub.2).sub.3 -- NH -- (CH.sub.2).sub.2 C.sub.6 H.sub.4 COOH residues. The enzyme retained by the carrier is then separated by a solution of ethyleneglycol. Use of the pure d-aminoacid oxidase for the preparation of cetonic acids.

REFERENCES:
patent: 3652761 (1972-03-01), Weetall
patent: 3746622 (1973-07-01), Nishikawa et al.

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