Purification of coenzyme A

Details Purification of coenzyme A Purification of coenzyme A

1973-07-31

1976-01-27

Naff, David M.

Textiles: fiber preparation

195 63, 195 66R, 195DIG11, 260112R, 2602115R, C07G 702, C07G 700, C07G 300

Patent

active

039350720

ABSTRACT:
Coenzyme A is purified by a process involving contacting a cyanogen halide-activated water-insoluble polysaccharide with a cell-free extract of a coenzyme A-producing microorganism to immobilize proteins such as enzymes having affinity for coenzyme A on the polysaccharide. The immobilized proteins are then contacted with a coenzyme A-containing solution to absorb coenzyme A followed by eluting coenzyme A from the immobilized proteins. Alternatively, the proteins having affinity for coenzyme A may be isolated from the cell-free extract prior to immobilization by absorbing the proteins on coenzyme A immobilized on a cyanogen halide-activated water-insoluble polysaccharide followed by eluting the proteins from the immobilized coenzyme A.

REFERENCES:
patent: 2828302 (1958-03-01), Mitz
patent: 3645852 (1972-02-01), Axen et al.
patent: 3834990 (1974-09-01), Werle et al.
wilchek et al., Conversion of Protein Kinase to a Cyclic AMP Independent Form by Affinity Chromatography on Ne-Caproyl 3',5'-Cyclic Adenosine Monophosphate-Seplharose. Biochemical and Biophysical Research Communications, Vol. 45, No. 5, 1971, (pp. 1177-1184).
Denburg et al., Purification of a Specific RNA by Sepharose -Round, Enzyme, Proceedings of the National Academy of Sciences, Vol. 67, No. 2, 1970, (pp. 1057-1062).

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