Purification of chimeric proteins containing an IL-2 moiety by r

Details Purification of chimeric proteins containing an IL-2 moiety by r Purification of chimeric proteins containing an IL-2 moiety by r

1988-10-24

1992-09-22

Draper, Garnette D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530351, 530402, 530403, 530404, 530405, 530406, 530409, 530410, 530411, 530412, 530413, 530810, 530811, 530812, 530813, 530814, 530417, 530422, 53038823, 435 71, 435 78, 435 691, 436501, 436518, 436524, 436527, 436528, 436529, 436530, 536 52, 536112, C07K 1500, C07K 320, C07K 1700, G01N 3350

Patent

active

051497883

ABSTRACT:
Methods are provided for the purification of interleukin-2 (IL-2) and chimeric proteins containing an IL-2 moiety from a wide variety of sources, including synthetic mixtures, cell culture conditioned medium and mammalian and bacterial recombinant expression systems. The methods of the invention employ IL-2 receptor-affinity adsorbents in which soluble IL-2 receptors have been immobilized on solid supports. Through the use of these affinity adsorbents, highly purified IL-2 and chimeric proteins containing an IL-2 moiety can be produced in a single step from bacterial extracts or conditioned medium. The proteins thus purified are largely free of aggregated forms, which are often present when other purification methods are used.

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