Purification of calcitonin by partition chromatography

Details Purification of calcitonin by partition chromatography Purification of calcitonin by partition chromatography

1980-09-22

1982-06-22

Phillips, Delbert R.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

C07C10352

Patent

active

043361870

ABSTRACT:
The purification of crude calcitonin by subjecting a crude calcitonin material to partition chromatography in which the solvent utilized is a mixture of butanol, a lower alphatic alcohol which is ethanol, methanol, or propanol, or mixtures thereof, acetic acid, water and ammonium acetate. The crude calcitonin of this invention is of a type known as ultimobronchial calcitonin. This includes calcitonins of the salmon and eel, whether the calcitonins of this type be obtained from natural sources or are prepared by synthesis. Preferably, the crude calcitonins of this type, which are purified by the process herein set forth, are obtained by solid phase synthesis in which the amino acid chain is assembled by attaching a resin support to the first amino acid, adding the amino acids one-by-one in the order in which they appear in the natural calcitonin; and finally removing the resin and the protective groups used in the synthesis.

REFERENCES:
patent: 3915949 (1975-10-01), Colescott et al.
FEBS Letters 65, (1976), 242-245.
Journal of Chromatography, 63 (1971), pp. 423-428, Elsevier Publishing Company, Amsterdam, The Netherlands.
Parathyroid Hormone and Thyrocalcitonin (Calcitonin); R. V. Talmage, L. F. Belanger and I. Clark, eds.; Excerpta Medica Foundation Offices, N.Y., pp. 43,68-73.
Merle, M., Lefevre, G. and Milhaud, G., Biochem. Biophy.; Res. Comm., 87, (2) pp. 455-460 (1979).
"A Proposed Structure for Rat Calcitonin", by P. G. H. Byfield, Jennifer L. McLoughlin, E. W. Matthews and I. MacIntyre; FEBS Letters; vol. 65, No. 2; Jun. 1976.

Affiliated with

Also associated with

Rating

Purification of calcitonin by partition chromatography does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of calcitonin by partition chromatography, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of calcitonin by partition chromatography will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-652389