Purification of apase-1.sub.1 and retrieval of the nematode resi

Details Purification of apase-1.sub.1 and retrieval of the nematode resi Purification of apase-1.sub.1 and retrieval of the nematode resi

1987-03-30

1990-06-12

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435815, C12N 918

Patent

active

049332864

ABSTRACT:
An isoenzyme from tomato, acid phosphatase-1 isoenzyme (Apase-1.sup.1), has ben purified to homogeneity and is subjected to amino acid sequencing and used to prepare anti-Apase-1 antibodies. The amino acid sequence permits design of probes to recover Apase-1.sup.1 -encoding cDNA; the antibodies are also useful for this purpose. The cDNA is useful to recover the genomic DNA encoding Apase-1.sup.1, which can then be used in walking or jumping techniques to recover the genomic DNA which confers nematode resistance, since this DNA resides immediately adjacent to the Apase-1.sup.1 gene on chromosome 6 of Lycopersicon esculentum.

REFERENCES:
Ozutsumi et al., "Immunological Properties of Two Acid Phosphatases from Tomato Fruit", Agr. Biol. Chem., vol. 47 (5) (1983), pp. 1137-1138.
Uehara et al., "Isolation of Violet-Colored Acid Phosphatase from Sweet Potato", J. Biochem, vol. 70 (1971), pp. 183-185.
Karger et al., An Introduction to Separation Science, John Wiley & Sons, N.Y., (1973), p. 338.
Medina-Filho, Ph.D. Thesis (1980) University of California, Davis.
Forbes et al., Tomato Genetics Cooperative Reports (1976) 26:7-8.
Rick et al., Tomato Genetics Cooperative Reports (1974) 24:25.
Rick, Genetic Maps (1984) 3:474 Cold Spring Harbor Press.
Bennetzen, Genetic Engineering (1984) pp. 491-512.
Collins et al., Proc. Natl. Acad. Sci. (1984) 81:6812-6816.
Martin et al., Nucleic Acids Research (1985) 13:8927-8938.
Poustka et al., Nature (1987) 325:353-355.
Poustka et al., TIC (1986) pp. 174-179.
Reyes et al, Genetic Engineering (1985) pp. 157-173.
Steinmetz et al., Nature (1982) 300:35-42.

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