Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1977-03-01
1979-12-25
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 424 12, 435180, 435803, 435 13, C07G 700, G01N 3100, G01N 3114
Patent
active
041804376
ABSTRACT:
Antiserum which has been generated against an enzyme-inhibitor-complex is purified by immunoprecipitating a freshly generated antiserum with fresh human bloodplasma to form a precipitate and a fluid, and subjecting the fluid to chromatographic separation over an insolubilized enzyme-inhibitor complex against which the antiserum has been generated. A reagent for immunochemical assay is prepared by contacting the purified antiserum with red bloodcells or synthetic resin particles to obtain on the surface of the red bloodcells or synthetic resin particles antibodies from the antiserum. In a diagnosis test, blood plasma is contacted with the purified antiserum and with red bloodcells or synthetic resin particles which bear the enzyme-inhibitor complex used to prepare the antiserum, and whether or not agglutination occurs is ascertained. In an alternative diagnosis test, blood plasma is contacted with red bloodcells or synthetic resin particles having on their surface antibodies from the antiserum, and whether or not agglutination occurs is ascertained.
REFERENCES:
patent: 3912805 (1975-10-01), Cayzer et al.
Collen, Thrombosis Research, vol. 5, pp. 777-779, 1974.
Collen et al., Thrombosis Research, vol. 7, pp. 235-238, 1975.
Eslinger Lewis H.
Leuven Research & Development v.z.w.
Naff David M.
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