Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Reexamination Certificate
2006-10-24
2006-10-24
Chan, Christina (Department: 1644)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
C530S395000
Reexamination Certificate
active
07125964
ABSTRACT:
A new method to capture, purify and expand antigen-specific T lymphocytes has been developed using magnetic beads coated with recombinant MHC class I molecules. This method was optimized using homogenous populations of naive T cells purified from mice transgenic for the 2C T cell receptor (TCR). These T cells were captured on beads coated with MHC class I molecules and the relevant antigenic peptides. MHC and peptide specificity was confirmed by the usage of irrelevant MHC peptide combinations. An enrichment of 800 to 1600 fold was measured, using 2C T cells mixed with irrelevant T cells, starting from a 2C T cell frequency of 1/3000. The same approach was used to purify antigen-specific CD8+T cells from total CD8+T cells from naive mice. The recovered cells could be expanded and specifically kill target cells in vitro; they had a significant effect in vivo as well. We expect this procedure to be suitable to purify and expand in vitro tumor- and virus-specific killer T cells for use in cell therapy.
REFERENCES:
patent: 5045320 (1991-09-01), Mescher
patent: 5521288 (1996-05-01), Linsley et al.
patent: 5529921 (1996-06-01), Peterson et al.
patent: 5583031 (1996-12-01), Stern
patent: 5595881 (1997-01-01), Kendrick et al.
patent: 5731160 (1998-03-01), Melief et al.
patent: 5827737 (1998-10-01), Peterson et al.
patent: 2 069 541 (1993-11-01), None
patent: 0 814 838 (1998-01-01), None
patent: WO 96/05287 (1996-02-01), None
patent: WO 96/27392 (1996-09-01), None
Burshtyn, DN et al. (1993)Journal of Immunology. 151:3070-3080.
Nikolic-Zugic, J et al. (1990) Eur. J. Immunol. 20:2431-2437.
Boyd, LF et al. (1992) Proc. Nat. Acad. Sci. (USA). 89:2242-2246.
Chang, KW et al. J. Biotech. [2005] 116:359-367.
Agrawal, G.B. et al., “Mathematical Modeling of Helper T Lymphocyte/Antigen-Presenting Cell Interactions: Analysis of Methods for Modifying Antigen Processing and Presentation”,J. Theor. Biol, 1996, 182, 487-504.
Ashman, R.F., “Lymphocyte Receptor Movement Induced by Sheep Erythrocyte Binding”,J. Immunol., 1973, 111, 212-220.
Altman, J.D., et al., “Phenotypic Analysis of Antigen-Specific T Lymphocytes”,Science, 1996, 274, 94-96.
Bellone, G. et al., “Regulatory Action of Prolactin on the In Vitro Growth of CD34+ Ve Human Hemopoietic Progenitor Cells”,J. Cell Physiol., 1995, 163, 221-231.
Borrow, P. et al., “Lymphocytic Choriomeningitis Virus”,Viral Pathogenesis, 1997, 593-627.
Brinkman, et al., “TCR-Independent Activation of Human CD4+45RO T Cells by Anti-CD38 Plus IL-2; Induction of Clonal Expansion and Priming for a Th2 Phenotype”,The Journal of Immunology, Jun. 1, 1996, 156, 4100-4106.
Cai, Z. et al., “Influence of Antigen Dose and Costimulation on the Primary Response of CD8+ I Cells in Vitro”,J. Exp. Med., 1996, 183, 2247-2257.
Cai, Z. et al., “TransfectedDrosophilaCells as a Probe for Defining the Minimal Requirements for Stimulating Unprimed CD8+ T Cells”,Proc. Natl. Acad. Sci. USA, 1996, 93, 14736-14741.
Corr, M. et al., “T-Cell Receptor-MHC Class I Peptide Interactions: Affinity, Kinetics, and Specificity”,Science, 1994, 265, 946-949.
DeBruijn, MLH. et al., “Mechanisms of Induction of Primary Virus Specific Cytotoxic T Lymphocyte Responses”,Eur. J. Immunol, 1992, 22, 3013-3020.
Dillon, Sr. et al., “Vβ5 T Cell Receptors Skew Toward OVA + H-2K b Recognition”,J. Immunol., 1994, 152, 1790-1801.
Engelhard VH., “Structure of Peptides Associated with MHC Class I Molecules”,Current Opinion Immunol., 1994, 6, 13-23.
Gold, MR. et al., “Biochemistry of B Lymphocyte Activation”,Adv. Immunol., 1994, 55, 221-295.
Grupp, SA. et al., “he Phosphatidylinositol Response is an Early Event in the Physiologically relevant Activation of Antigen-Specific B Lymphocytes”,Cell. Immunol.
Hou, S. et al., “Virus-Specific CD8+ T-Cell memory Determined by Clonal Burst Size”,Nature, 1994, 369, 652-654.
Irsch, J. et al., “Switch Recombination in Normal IgA1+B Lymphocytes”,Proc. Natl. Acad. Sci. USA, 1994, 91, 1323-1327.
Jackson, MR. et al., “Empty and Peptide-Containing Conformers of Class I Major Histocompatibility Complex Molecules Expressed inDrosophila melanogasterCells”,Proc. Natl. Acad. Sci. USA, 1992, 89, 12117-12121.
Kane, KP. et al., “Class I Alloantigen is Sufficient for Cytolytic T Lymphocytes Binding and Transmembrane Signaling”,Eur. J. Immunol, 1988, 18, 1925-1929.
Kane, K.P. et al., “Activation of CD-8-Dependent Cytotoxic T Lymphocyte Adhesion and Degranulation by Peptide Class I Antigen Complexes”,J.Immunol.,1993, 150, 4788-4797.
Kato, K. et al., “Isolation and Characterization of CD34+ Hematopoietic Stem cells from Human Peripheral Blood by High Gradient Magnetic Cell Sorting”,Cytometry, 1993, 14, 384-392.
Klavinskis, LS. et al., “Efficiency and Effectiveness of Cloned Virus-Specific Cytotoxic T Lymphocytes in Vivo”,J. Immunol, 1989, 143, 2013-2016.
Kranz, DM. et al., “Attachment of an Anti-receptor Antibody to Non-Target Cells Renders them Susceptible to Lysis by a Clone of Cytotoxic T Lymphocytes”,Proc. Natl. Acad. Sci USA, 1994, 81, 7922-7926.
Lau, L.L. et al, “Cytotoxic T-Cell Memory Without Antigen”,Nature, 1994, 369, 648-652.
Luxembourg, A.T. et al., “Modulation of Signaling via the B Cell Antigen Receptor by CD21, the Receptor for C3dg and EBV”,J. Immunol., 1994, 153, 4448-4467.
Matsui, K.et al., “Low Affinity Interaction of Peptide-MHC Complexes with T Cell Receptors”,Science, 1991, 254, 1788-1791.
Mescher, M.F., “Molecular Interactions in the Activation of Effector and Precursor Cytotoxic T Lymphocytes”,Immunol. Rev., 1995, 146, 177-210.
Moore, M.D. et al., “Hydrodynamic, Electron Microscopic, and Ligand-Binding Analysis of the Epstein-Barr Virus/C3dg Receptor(CR2)”,J. Biol. Chem., 1989, 264, 20576-20582.
Myers, C.D. et al., “Antigen-Induced Changes in Phospholipid Metabolism in Antigen-Binding B Lymphocytes”,J. Immunol., 1987, 138, 1705-1711.
Nakanishi, M. et al., “Binding of Cytotoxic T-Lymphocytes to Supported Lipid Monolayers Containing Trypsinizesd H-2K k”,Mol. Immunol., 1983, 20, 1227-1231.
Noelle, R.J. et al., “Cognate Interactions between Helper T Cells and B Cells”,Immunol Today, 1990, 11, 361-368.
Oehen, S. et al., “Antivirally Protective Cytotoxic T Cell Memory to Lymphocytic Choriomeningitis Virus is governed by Persisting Antigen”,J. Exp. Med., 1992, 176, 1273-1281.
Radbruch, A. et al., “Detection and Isolation of Rare Cells”,Curr. Opinion Immunol., 1995, 7, 270-273.
Ramensee, H.G. et al., “MHC Ligands and Peptide Motifs: First Listing”,Immunogenetics, 1995, 41, 178-228.
Sawada, K. et al., “Purification of Human Blood Burst-forming Units-Erythroid and Demonstration of the Evolution of Erythropoietin Receptors”,J. Cell. Physiol., 1990, 142, 219-230.
Sha, W.C. et al., “Selective Expression of an Antigen Receptor on CD8 Bearing T Lymphocytes in Transgenic Mice”,Nature, 1988, 335, 271-274.
Snow, E.C. et al., “Activation of Antigen-Enriched B Cells. II. Role of Linked Recognition in B Cell Proliferation to thymus-Dependant Antigens”,J. Immunol., 1983, 130, 614-618.
Snow, E.C. et al., “Induction of the c-myc Protooncogene after Antigen Binding to Hapten-Specific B Cells”,J. Exp. Med., 1986, 164, 944-949.
Snow, E.C. et al., “Activation of Antigen-Enriched B Cells”,The Journal of Immunolog, Feb. 1983, 130(2).
Stein, P. et al., “Induction of Antigen-Specific Proliferation in Affinity-Purified Small Lymphocytes: requirement for BSF-1 by Type 2 but not Type 1 thymus-Independent Antigens”,J. Immunol, 1986, 136, 2080-2089.
Sun, S. et al., “Dual Function of Drosophila Cells as APCs for Naïve CD8+ t Cells: implications for Tumor Immunothe
Jackson Michael R.
Luxembourg Alain T.
Peterson Per A.
Chan Christina
Ortho-McNeil Pharmaceutical , Inc.
VanderVegt F. Pierre
Woodcock & Washburn LLP
LandOfFree
Purification of antigen-specific T cells does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification of antigen-specific T cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of antigen-specific T cells will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3642891