Purification of antibodies

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530413, 530417, 530412, 424 858, 435803, C07R 312

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active

048410241

ABSTRACT:
I(g)G3 antibody is purified by affinity chromatography and collection of released antibody at a pH of 9.0 to 9.6. The purification is effected in a column containing both an affinity matrix and a desalting matrix, with the column being equilibrated to a pH of from 9.0 to 9.6. I(g)G3 antibody may be stored at pH 9.0 to 9.6.

REFERENCES:
patent: 4233286 (1980-11-01), Soothill et al.
patent: 4272506 (1981-06-01), Schwarzberg
patent: 4560504 (1985-12-01), Arnold
patent: 4618589 (1986-10-01), Jifferie et al.
patent: 4639513 (1987-01-01), Hou et al.

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