Purification of .alpha.-1 proteinase inhibitor using novel chrom

Details Purification of .alpha.-1 proteinase inhibitor using novel chrom Purification of .alpha.-1 proteinase inhibitor using novel chrom

1994-08-24

1997-03-11

Fleisher, Mindy

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530380, 530392, 530395, 530830, C07K 116, C07K 14435, A61K 3514

Patent

active

056102852

ABSTRACT:
Cation chromatography with solutions at pH less than 6.0 and low ionic strength can be utilized to purify human .alpha.-1 proteinase inhibitor (.alpha.-1 PI) from biological fluids including plasma and plasma fractions. The cation chromatography takes advantage of the fact that active .alpha.-1 PI does not bind to the cation column under these conditions but other proteins, including denatured .alpha.-1 PI and albumin, do. The effect is that active .alpha.-1 PI flows through the chromatography column leaving the contaminating proteins behind. Recovery of .alpha.-1 PI is high and improvement of purity is dramatic.

REFERENCES:
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patent: 4379087 (1983-04-01), Coan et al.
patent: 4629567 (1986-12-01), Bollen et al.
patent: 4656254 (1987-04-01), Shearer et al.
patent: 4752576 (1988-06-01), Brake et al.
patent: 5319072 (1994-06-01), Uemora et al.

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