Purification of acidic proteins using ceramic hydroxyapatite...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C424S177100, C530S415000

Reexamination Certificate

active

08058407

ABSTRACT:
The present invention provides a method of removing product-related inactive or partially active species, high molecular weight aggregates, as well as other process-related impurities from preparations of acidic proteins by using ceramic hydroxyapatite chromatography.

REFERENCES:
patent: 5225538 (1993-07-01), Capon et al.
patent: 5399677 (1995-03-01), Wolfman et al.
patent: 5428130 (1995-06-01), Capon et al.
patent: 5455165 (1995-10-01), Capon et al.
patent: 5514582 (1996-05-01), Capon et al.
patent: 5516964 (1996-05-01), Umansky et al.
patent: 5633162 (1997-05-01), Keen et al.
patent: 5714147 (1998-02-01), Capon et al.
patent: 5874247 (1999-02-01), Toyoshima et al.
patent: 6136310 (2000-10-01), Hanna et al.
patent: 7094604 (2006-08-01), Snyder et al.
patent: 7122641 (2006-10-01), Vedantham et al.
patent: 2003/0165496 (2003-09-01), Basi et al.
patent: 2005/0107594 (2005-05-01), Sun et al.
patent: 2009/0186396 (2009-07-01), Gagnon
patent: 2009/0187005 (2009-07-01), Gagnon
patent: 0433225 (1991-06-01), None
patent: WO03/059935 (2003-07-01), None
patent: WO 2004/076485 (2004-09-01), None
patent: WO 2005/044856 (2005-05-01), None
Gorbunoff, M., Protein Chromatography on Hydroxyapatite Columns, Methods in Enzymology, vol. 117, Academic, Press, Inc., 1985, pp. 370-380.
“Macro-Prep Chromatography Supports”; Bio-Rad, 8 pages.
Ettinger et al., “Interleukin 21 as a Target of Intervention in Autoimmune Disease”, Ann Rheum Dis; 67 (Suppl III):iii83-iii86 (2008).
Franklin, S., “Removal of Aggregate From an IgG4 Product Using CHT Ceramic Hydroxyapatite”, Chromatography Tech Note 2940, Bio-Rad Laboratories, Inc. (2002).
Gagnon, “Hydroxyapatite Chromatography”, Purification Tools for Monoclonal Antibodies, Validated Biosystems, Chapter 5, p. 87-102 (1996).
Gorbunoff, “Protein Chromatography on Hydroxyapatite Columns”, Methods Enzymol. 182:329-39 (1985).
Karlsson et al,“Ion Exchange Chromatography”, Protein Purification (VCH Publishers, Inc., New York NY) pp. 107-148 (1989).
Kennedy, “Hydrophobic-Interaction Chromatography”, Current Protocols in Protein Sciences, 8.4.1-8.4.21 (1995).
Kohno, “Refolding of Recombinant Proteins”, Meth. Enzymol. 185:187-95 (1990).
Lullau et al, “Develpoment of a Bioprocess for Murine Dimeric IgA Production”, Biotechnology Techniques 12(6):425-430 (1998).
Ogawa et al, “Effect of pH on Gradient Elution of Different Proteins on Two Types of Ceramic Hydroxyapatite”, American Laboratory 171-17K (1996).
Shepard et al.,“Discoloration of Ceramic Hydroxyapatite Used for Protein Chromatography”, Journal of Chromatography A 891:93-98 (2000).
Sinacola et al,, “Rapid Refolding and Polishing of Single-chain Antibodies fromEscherichia coliInclusion Bodies”, Protein Expression and Purification 26:301-308 (2002).
Stanker et al., “One-Step Purification of Mouse Monoclonal Antibodies from Ascites Fluid by Hydroxylapatite Chromatography”, Journal of Immunological Methods 76:157-169 (1985).
Steindl, F et al. “A Simple Methods to Quantify Staphyloccal Protein A in the Presence of Human or Animal IgG in Various Samples”, J. Immunol. Methods 235:61-69 (2000).
Tarditi,et al., “Selective High-Performance Liquid Chromatographic Purification of Bispecific Monoclonal Antibodies”, J. Immunol. Methods 599:13-20 (1992).
Usami et al., “The Effect of pH, Hydrogen Peroxide and Temperature on the Stability of Human Monoclonal Antibody”, Journal of Pharmaceutical and Biomedical Analysis 14:1133-1140 (1996).
Vola et al. “Comparision of Two Different HPLC Hydroxylapatite Matrices for Resolution of IgG Idiotypes”, BioTechniques 14:650-655 (1993).
Williams et al., “Ion-Exchange Chromatography”, Current Protocols in Protein Sciences, p. 8.2.1-8.2.30 (1999).
Yazaki et al., “Mammalian Expression and Hollow Fiber Bioreactor Production of Recombinant Anti-CEA Diabody and Minibody for Clinical Applications”, Journal of Immunological Methods 253; 195-208 (2001).
Coppola et al. “High-Performance Liquid Chromatography of Amino Acids, Peptides and Proteins”, Journal of Chromatography 476:269-290 (1989).
Giovannini et al. “Comparison of Different Types of Ceramic Hydroxyapatite for the Chromatographic Separation of Plasmid DNA and a Recombinant Anti-Rhesus D Antibody”, Bio Sep 9:359-368 (2001).
Josic et al. “Purification of Monoclonal Antibodies by Hydroxylapatite HPLC and Size Exclusion HPLC”, Biological Chemistry Hoppe-Seyler 372:149-156 (1991).
Stevens et al. “Techniques for Purifying Monoclonal Antibodies”, American Biotechnology Laboratory 3(5):22-30 (1985).
Biorad CHT Ceramic hydroxyapatite Product Information Sheet, p. 1-4 (2002).
International Search report and Written Opinion for PCT/US2009/062528 Dec. 16, 2009.
Giovannini et al., “Isolation of a Recombinant Antibody from Cell Culture Supernatant: Continuous Annular Versus Batch and Expanded-Bed Chromatography”, Biotechnology and Bioengineering 73:522-529 (2001).
Jungbauer, A., et al “Comparison of Protein A, Protein G and Copolymerized Hydroxyapatite for the Purification of Human Monoclonal Antibodies”, Journal of Chromatography 476:257-268 (1989).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Purification of acidic proteins using ceramic hydroxyapatite... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification of acidic proteins using ceramic hydroxyapatite..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification of acidic proteins using ceramic hydroxyapatite... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4281047

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.