Purification of 12-O-tetradecanoylphorbol-13-acetate, phorbol, a

Details Purification of 12-O-tetradecanoylphorbol-13-acetate, phorbol, a Purification of 12-O-tetradecanoylphorbol-13-acetate, phorbol, a

1983-01-17

1984-08-28

Adee, John

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 210658, B01D 1508

Patent

active

044683286

ABSTRACT:
Droplet counter-current chromatography is utilized to isolate and purify 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol, and the diastereoisomer 4.alpha.-phorbol from croton oil. The stationary and mobile phases for the procedure are derived from an equilibrated mixture of hexane-diethylether-n-propanol-ethanol-water, the separated upper layer of which comprises the stationary phase and the bottom layer of which the mobile phase. The TPA and the phorbol isomers are obtained in exceptionally high purity by further treatment with liquid column chromatography and thin-layer chromatography.

REFERENCES:
patent: 3962074 (1976-06-01), Schrom
patent: 3996132 (1976-12-01), Mateos
Thin-Layer Chromatography An Annotated Bibliography: 1964-1968 by Haywood. Ann Arbor Science Pub. Ann Arbor, Mich. p. 261, 1968.
Isolation of Phorbal and 4.alpha.-Phorbol from Croton Oil by Marshall and Kinghorn in Journal of Chromatography 206(1981)421-424.

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