Purification method of human interferon

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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424 85, 435 68, 435811, A61K 4502

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045419523

ABSTRACT:
A method for the purification of human interferon by the successive treatments of a crude interferon solution with an immobilized carrier which is coupled with a blue dye and a chelating carrier which contains a chelating residue chelated with at least one metal ion selected from the group consisting of Co.sup.2+, Ni.sup.2+ and Zn.sup.2+.

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patent: 4289689 (1981-09-01), Friesen
patent: 4359389 (1982-11-01), Heine
Colowick and Kaplan, Eds.: Methods in Enzymology, "Interferons", Part A, vol. 78, Pestka, Ed. Academic Press, 1981.
W. J. Jankowski et al., Biochemistry, 15, 5182-5187 (1976).
E. Bollin et al., Preparative Biochemistry, 8, 259-274 (1978).
J. S. Erickson et al., Archives of Virology, 63, 253-261 (1980).
E. Knight, Jr., et al., Science, 207, 525-526 (1980).
H. Friesen et al., Archives of Biochemistry and Biophysics, 206, 432-450 (1981).
V. G. Edy et al., J. Biol. Chem., 252, 5934-5935 (1977).
K. C. Chada et al., J. Gen. Virol., 43, 701-706 (1979).
J. W. Heine et al., J. Gen. Virol., 54, 47-56 (1981).
Berg et al., The Complete Purification of Human Leucocyte Interferon, Scand. J. Immunol., 11, pp. 489-502, 1980.

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