Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1985-10-30
1987-06-30
Welsh, Maurice J.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530351, 530416, 435 68, C07K 320
Patent
active
046771972
ABSTRACT:
An improved method for purifying TNF, especially recombinantly produced TNF in bacteria, is disclosed. The purification employs a hydrophobic support in a chromatographic column which is then developed, preferably by HPLC. Preliminary purifications using anion exchange resins are also helpful.
REFERENCES:
patent: 4289690 (1981-09-01), Pestka et al.
patent: 4309418 (1982-01-01), Green
patent: 4495282 (1985-01-01), Ohnishi et al.
Shirai et al., "Cloning and Expression in E. Coli . . . TNF", Nature 313 1985, p. 803.
Wang et al., "Molecular Cloning of the Complementary DNA for h-TNF", Science, vol. 228, 1985, p. 149.
Pennica et al., "Human Tumour Necrosis Factor", Nature 312 (5996) 1985, p. 724.
Abe et al., "Purification of Rabbit Tumor Necrosis Factor", FEB, 1985, vol. 180(2), pp. 203-206.
Williamson et al., PNAS, 80, 5397-5401 (1983).
Lin Leo S.
Yamamoto Ralph
Cetus Corporation
Draper Garnette D.
Fineman Elliott L.
Hasak Janet E.
Murashige Kate H.
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