Purification method

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai

Reexamination Certificate

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Details

C514S002600, C530S384000, C530S395000, C530S412000, C530S416000, C424S078100, C424S078110

Reexamination Certificate

active

06992061

ABSTRACT:
The invention relates to a method of removing endotoxin from preparation of alpha-1-acid glycoprotein (orosomucoid) by contact with a finely divided non-toxic resin such as fumed silica. The invention also relates to a purification process for alpha-1-acid glycoprotein which includes this depyrogenation step, and to the depyrogenated product and its clinical uses.

REFERENCES:
patent: 3998946 (1976-12-01), Condie et al.
patent: WO 95/07703 (1995-03-01), None
patent: 95/22556 (1995-08-01), None
Botten et al., European Juornal of Immunology, vol. 22, No. 10, pp. 2687-2695, Oct. 1992.
Libert et al., J. Exp. Med., vol. 180, pp. 1571-1575, Oct. 1994.
Boutten, A., et al., “Alpha1-Acid Glycoprotein Potentiates Lipopolysaccharide-induced Secretion of Interleukin-1 Beta, Interleukin-6 and Tumor Necrosis Factor-alpha by Human Monocytes and Alveolar and Peritoneal Macrophages”,European Journal of Immunology, 22(10), pp. 2687-2695, (Oct. 1992).
Chen, R.F., “Removal of Fatty Acids from Serum Albumin by Charcoal Treatment”,The Journal of Biological Chemistry, 242(2), pp. 173-181, (Jan. 25, 1967).
Edwards, C.A., et al., “Tri (n-Butyl) Phosphate/Detergent Treatment of Licensed Therapeutic and Experimental Blood Derivativesl”,International Journal Vox Sanguinis of Transfusion Medicine, 52, pp. 53-59, (1987).
Hao, Y., et al., “Development of Large-Scale Fractionation Methods IV. A Simple Method For the Large-Scale Preparation of alpha1-Acid Glycoprotein”,Biochimica Et Biophysica Acta(P),322(1), pp. 99-108, (Sep. 21, 1973).
Kistler, P., et al., “Large Scale Production of Human Plasma Fractions”,Vox Sanguinis, 7, pp. 414-424, (1962).
Libert, C., et al., “Protection by alpha1-acid Glycoprotein Against Tumor Necrosis Factor-induced Lethality”,J. Exp. Med., 180, 1571-1575, (Oct. 1994).
Pentel, P.R., et al., “Effects of High Dose Alpha-1-Acid Glycoprotein on Despiramine Toxicity in Rats”,Journal of Pharmacology and Experimental Therapeutics, 246(3), 1061-1066, (1988).
Weary, M., “Depyrogenation, in”,PYROGENS: Endotoxins, LAL Testing, and Depyrogenation, Frederick C. Perason ed., Marcel Dekker, Inc., 203-218, (1985).
Keyler, Daniel E., “Effects of Alpha-1-Acid Glycoprotein Administration on Propranolol Binding and Beta Blockade in Rats”,Biochemical Pharmacology, vol. 38, No. 7, (1989),1163-1168.
Kremer, Josephina M., “Drug Binding to Human Alpha-1-acid Glycoprotein in Health and Disease”,Pharmacological Reviews, vol. 40, No. 1, (1988),1-47.
Seaberg, David C., “Effects of Alpha-1-acid Glycoprotein on the Cardiovascular Toxicity of Nortriptyline in a Swine Model”,Vet Hum Toxicol, vol. 33, No. 3, (Jun. 1991),226-231.

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