Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-01-26
1996-10-29
Rollins, John W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435100, 435183, 435232, 435822, 435850, C12N 900, C12N 952, C12N 120
Patent
active
055696007
ABSTRACT:
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Monoclonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
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Cooney Charles L.
Langer Robert S.
Linhardt Robert J.
Lohse Daniel L.
Sasisekharan Ramnath
Massachusetts Institute of Technology
Rollins John W.
University of Iowa Research Foundation
Ware Deborah K.
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