Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-09-05
2000-10-03
Eisenschenk, Frank C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 68, 435 694, 435 695, 435 701, 435195, 530350, 530351, 530385, 530399, 530412, 530415, C12P 2106, C12P 2104, C07K 100, A61K 3824
Patent
active
061271500
ABSTRACT:
The present invention is directed to a protein purification construct having three tandem, coupled segments composed of a binding protein, an interconnecting linker and a variable fused polypeptide which incorporates the one or more copies of a product peptide. The binding protein is a mammalian or human carbonic anhydrase, or a modified version of the carbonic anhydrase. The protein purification construct may be employed in methods for expression of the product peptide in microbial and higher organism and for ligand immobilized affinity purification of the product peptide.
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Coolidge Thomas R.
Heeke Gino Van
Schuster Sheldon M.
Stout Jay
Wagner Fred
BioNebraska, Inc.
Eisenschenk Frank C.
Zeman Mary K
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