Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1991-01-18
1993-12-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 691, 935 14, 536 232, C12N 1500
Patent
active
052684652
ABSTRACT:
A method of purifying calmodulin-dependent nitric oxide synthase provides a homogeneous preparation of the enzyme. The enzyme is used to raise antibodies which are a useful immunohistochemical reagent. The antibodies localize calmodulin-dependent nitric oxide synthase to a number of anatomical sites, including retina, intestine, adrenal gland, and vasculature. However, activated macrophages, which are known to possess a nitric oxide producing activity, do not display an immunoreactive protein of appropriate size on Western blots using the antibodies. Nucleotide sequences encoding calmodulin-dependent nitric oxide synthase indicate a novel sequence with a flavin binding site consensus sequence.
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Bredt David S.
Snyder Solomon H.
Moore William W.
The Johns Hopkins University
Wax Robert A.
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