Purification and manipulation of bone marrow and blood cells on

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 2, 435 721, 4352402, 436 56, 436172, 436536, G01N 3349, G01N 33533, G01N 33536

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active

054647533

ABSTRACT:
The invention provides methods for purifying or manipulating bone marrow and blood cells based upon P-glycoprotein expression. The methods rely upon immunopurification procedures or upon differential accumulation of materials subject to P-glycoprotein mediated efflux.

REFERENCES:
patent: 5061620 (1991-10-01), Tsukamoto et al.
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Efferth et al, "Reciprocal Correlation Between Expression of P-Glycoprotein and accumulation of Rhodamine 123 in Human Tumors", Anticancer Research vol. 9, pp. 1633-1638, 1989.
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Mulder et al, "Separation and Functional Analysis of Bone Marrow Cells Separated by Rhodamine-123 Fluorescence", Exp. Hematol, vol. 15, pp. 99-104, 1987.
Bertoncello et al, "Multiparameter Analysis of Transplantable Hemopoietic Stem Cells: 1. The Separation and Enrichment of Stem Cells Homing to Marrow and Spleen on the Basis of Rhodamine-123 Fluorescence", Exp. Hematol. vol. 13, pp. 999-1006, 1985.
van der Bliek et al., "Multi Drug Resistance", Advances in Cancer Research 52:165 1989.
Neyfakh 1988. "Use of Fluorescent Dyes as Molecular Probes for the Study of Multidrug Resistance", Exp. Cell Res. 174:168-176.
Hamada et al., "Mouse-Human Chimeric Antibody against the Multidrug Transporter P-Glycoprotein", Jun. 1990. Cancer Res 50:3167.

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