Purification and immobilization of sulfhydryl oxidase

Details Purification and immobilization of sulfhydryl oxidase Purification and immobilization of sulfhydryl oxidase

1975-10-10

1978-05-02

Naff, David M.

Textiles: fiber preparation

195 66R, 195 68, 195DIG11, 195116, 426 42, 4263302, 426522, C12D 1310, C07G 702, A23C 302, A23C 912

Patent

active

040873285

ABSTRACT:
Sulfhydryl oxidase is isolated in substantially purified form by precipitating from whey a crude impure fraction of sulfhydryl oxidase, dissolving the crude sulfhydryl oxidase in a dilute neutral buffer solution, equilibrating to allow for dissociation of the dissolved sulfhydryl oxidase, separating the equilibrated sulfhydryl oxidase from molecularly larger materials, concentrating the resultant separated sulfhydryl oxidase and separating the concentrated sulfhydryl oxidase from molecularly smaller materials to isolate a substantially purified sulfhydryl oxidase enzyme having a specific activity at least about fifty times greater than the crude enzyme and about 3000 times greater than that in skim milk. The enzyme may be immobilized, for example, by attaching it to an inert support. Activity of the immobilized enzyme may be regenerated by treatment with an aqueous solution of ferrous ions.

REFERENCES:
patent: 2482724 (1949-09-01), Baker
patent: 2788283 (1957-04-01), Stewart, Jr. et al.
patent: 3282702 (1966-11-01), Schreiner
patent: 3627640 (1971-12-01), Blumberg et al.
patent: 3705084 (1972-12-01), Reynolds
Swaisgood et al., Immobilization of Sulfhydryl Oxidase and some of its Kinetic Properties, J. Da. Sci., vol. 58, No. 5, May 1975 (p. 796).
Janolino, et al., Isolation and Characterization of Sulfhydryl Oxidase from Bovine Milk, The Journal of Biological Chemistry, vol. 250, No. 7, 4/10/75 (pp. 2532-2538).

Affiliated with

Also associated with

Rating

Purification and immobilization of sulfhydryl oxidase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Purification and immobilization of sulfhydryl oxidase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification and immobilization of sulfhydryl oxidase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2157177