Purification and administration of DNA repair enzymes

Details Purification and administration of DNA repair enzymes Purification and administration of DNA repair enzymes

1988-07-06

1991-12-31

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435196, 435199, 424 945, 424 946, 424450, C12N 910, C12N 916, A61K 3752, A61K 3754

Patent

active

050772112

ABSTRACT:
Methods for purifying DNA repair enzymes are provided in which an aqueous solution of a DNA repair enzyme in an impure state is applied to a molecular sieve column having an exclusion limit which will retard the DNA repair enzyme but will not retard contaminants larger than the DNA repair enzyme. The DNA repair enzyme in an enhanced state of purity is eluted isocratically from the molecular sieve column in an elution buffer and applied directly to a DNA affinity column in the same buffer without intermediate dialysis, ultrafiltration, or other procedures. The DNA repair enzyme is eluted from the DNA affinity column using, for example, a salt gradient. The method is rapid, inexpensive, simple to perform, and has been found to produce a homogeneous final product. In accordance with other aspects of the invention, the purified DNA repair enzymes are encapsulated in liposomes and administered to living cells in situ. This form of administration has been found to be non-toxic to the cells and to result in increased incision of damaged DNA, enhanced DNA repair synthesis, and increased cell survival after exposure to ultraviolet light.

REFERENCES:
patent: 4356167 (1982-10-01), Kelly
patent: 4789633 (1988-12-01), Huang et al.
Myrnes et al., Eur. J. Biochem., vol. 173, No. 2, pp. 383-387, 1988.
Michael Mezei and Vijeyalakshmi Gulasekharam, Life Sciences, vol. 26, pp. 1473-1477, 1980.
Michael Mezei and Vijeyalakshmi Gulasekharam, Journal of Pharmacy and Pharmacology, vol. 34, pp. 473-474, 1981.
Adrienn Gesztes and Michael Mezei, Anesthesia and Analgesia, vol. 67, pp. 1079-1081, 1988.
H. M. Patel, Biochemical Society Transactions, vol. 13, pp. 513-516, 1985.
W. Wohlrab and J. Lasch, Dermatologica, vol. 174, pp. 18-22, 1987.
Robert Straubinger, Keelung Hong, Daniel Friend and Demetrios Papahadjopoulos, Cell, vol. 32, pp. 1069-1079, 1983.
Robert Straubinger, Nejat Duzgunes and Demetrios Papahadjopoulos, FEBS Letters, vol. 179, pp. 148-154, 1985.
Chaper 11 of the book Cell Fusion, edited by A. E. Sowers, Plenum Press, N.Y., 1987, pp. 241-267.
Ellens, Bentz and Szoka, Biochemistry, vol. 23, pp. 1532-1538, 1984.
Bentz, Ellens and Szoka, Biochemistry, vol. 26, pp. 2105-2116, 1987.
Ganesan, et al., International Journal of Pharmaceutics, vol. 20, pp. 139-154, 1984.
Ho, et al., Journal of Controlled Release, vol. 2, pp. 61-65, 1985.
Jacobs, et al., J. Pharm. Pharmacol., vol. 40, pp. 829-833, 1988.
Komatsu, et al., Chem. Pharm. Bull., vol. 34, pp. 3415-3422, 1986.
Shek, et al., Biochimica et Biophysica Acta, vol. 902, pp. 229-236, 1987.
Strauss, G., J. Soc. Cosmet. Chem., vol. 40, pp. 51-60, 1989.
Vermorken, et al., J. Pharm. Pharmacol., vol. 36, pp. 334-336, 1984.
Westerhof, W., Medical Hypotheses, vol. 16, pp. 283-288, 1985.
Liposome Technology, edited by G. Gregoriadis, 1984, published by CRC Press, Boca Raton, Florida, vol. II, Chapter 12, pp. 177-185.
Liposome Technology, edited by G. Gregoriadis, 1984, published by CRC Press, Boca Raton, Florida, vol. II, Chapter 15, pp. 207-221.
Repair of DNA Lesions Introduced by N-Nitroso Compounds, B. Myrnes & H. Krokan eds., Norwegian Univ. Press, Oslo, 1986, pp. 112-134.
Yamaizumi et al., Mutation Research, vol. 217, pp. 135-140, 1989.
Chenevert et al., Molecular and General Genetics, 1986, vol. 203, pp. 163-171.
Ciarrocchi et al., Proceedings of the National Academy of Sciences U.S.A., 1978, vol. 75, pp. 1887-1891.
Demple et al., The Journal of Biological Chemsitry, vol. 257, pp. 13776-13780, 1982.
Freeman et al., Analytical Biochemistry, vol. 158, pp. 119-129, 1986.
Friedberg et al., Journal of Bacteriology, 1971, vol. 106, pp. 500-507.
Higgins et al., Mutation Research, 1987, vol. 183, pp. 117-121.
Hoeijmakers, Journal of Cell Science Suppl., 1987, vol. 6, pp. 111-125.
McMillan et al., Journal of Virology, 1981, vol. 40, pp. 211-223.
Mosmann, Journal of Immunological Methods, vol. 65, pp. 55-63, 1983.
Myrnes et al., Carcinogenesis, 1984, vol. 5, pp. 1061-1064.
Nakabeppu et al., The Journal of Biological Chemistry, vol. 260, pp. 7281-7288, 1985.
Nakabeppu et al., Proceedings of the National Academy of Sciences, 1981, vol. 78, pp. 2742-2746.
Nakabeppu et al., Journal of Biological Chemistry, 1982, vol. 257, pp. 2556-2562.
Ostro, "Liposomes", Scientific American, Jan. 1987, vol. 256, pp. 2-11.
Robbins et al., Annals of Internal Medicine, vol. 80, No. 2, pp. 221-248, Feb., 1974.
Seawell et al., in DNA Repair, edited by Friedberg et al., NY, 1981, vol. 1, Part A, pp. 229-236.
Tanaka et al., Proceedings of the National Academy of Sciences, U.S.A., 1977, vol. 74, pp. 2958-2962.
Tanaka et al., Proceedings of the National Academy of Sciences U.S.A., 1975, vol. 72, pp. 4071-4075.
Valerie et al., Cancer Research, 1987, vol. 47, pp. 2967-2971.
Warner et al., Journal of Virology, 1981, vol. 40, pp. 204-210.
Yarosh, Mutation Research, vol. 145, pp. 1-16, 1985.
Yarosh et al., Molecular and Cellular Biology, 1981, vol. 1, pp. 237-244.
Yasuda et al., Proceedings of the National Academy of Sciences, Dec., 1970, vol. 67, pp. 1839-1845.
Yasuda et al., Biochimica et Biophysica Acta, 1976, vol. 442, pp. 197-207.
DNA Repair, edited by Friedberg et al., vol. I, Part A, Chapters 18, 20-22; vol. II, Chapters 3-5; vol. III, Section IV, Chapters 23-25.

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