Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1984-06-01
1985-05-21
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
424 85, 435 68, 435 70, 4351723, 435240, 435241, 435948, 436548, C07G 700
Patent
active
045185263
ABSTRACT:
A process for isolating and purifying a precipitated heterologous protein from a host cell culture, including treating the host cell culture with a buffered solution of ionic strength suitable to solubilize most of the host protein but in which refractile heterologous protein is substantially insoluble, and disrupting the cells to form a supernatant and an insoluble fraction; treating the insoluble fraction with a strongly denaturing solution to solubilize the refractile heterologous protein; and recovering renatured heterologous protein.
REFERENCES:
patent: 4414150 (1983-11-01), Goeddel
patent: 4438032 (1984-03-01), Golde et al.
patent: 4462940 (1984-07-01), Hanisch et al.
Goeddel et al., Nature, vol. 281, pp. 544 to 548 (Oct. 18, 1979).
Kleid et al., Science, vol. 214, pp. 1125 to 1129 (Dec. 4, 1981).
Williams et al., Science 215, 687-688 (1982).
Goeddel et al., Proc. Natl. Acad. Sci. USA 76, 106-110 (1979).
Wetzel et al., Biochemistry 19, 6096-6104 (1980).
Wetzel et al., Gene 16, 63-71 (1981).
Ullmann et al., Biochem. Biophys. Res. Commun. 35, 35-42 (1969).
Genentech Inc.
Schain Howard E.
LandOfFree
Purification and activity assurance of precipitated heterologous does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Purification and activity assurance of precipitated heterologous, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Purification and activity assurance of precipitated heterologous will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1537248